Kết quả gây nhiễm giun lươn cho lợn và đặc điểm lâm sàng ở lợn bệnh=Results of eelworm experimental infection in pig and clinical features in infected pigs

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Tác giả: Đình Nguyên Nguyễn, Thị Hương Nguyễn, Thị Hương Giang Nguyễn, Thị Kim Lan Nguyễn, Đức Hoàn Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2024

Mô tả vật lý: tr.68

Bộ sưu tập: Báo, Tạp chí

ID: 486414

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Chúng tôi tiến hành gây nhiễm giun lươn cho 15 con lợn khỏe mạnh 1 tháng tuổi bằng phương pháp phân lô thí nghiệm, gồm 3 lô (lô 1: gây nhiễm giun lươn cho lợn qua da, lô 2: gây nhiễm giun lươn cho lợn qua đường tiêu hóa và lô 3: đối chứng - không gây nhiễm giun lươn), đồng thời theo dõi diến biến lâm sàng của 1.265 lợn nhiễm giun lươn tự nhiên, nhằm xác định thời gian hoàn thành vòng đời của giun lươn và những đặc điểm lâm sàng của lợn mắc bệnh. Mầm bệnh gây nhiễm được nuôi như sau: Trứng giun lươn được thu thập từ phân và chất chứa đường tiêu hóa của lợn nhiễm với cường độ rất nặng, các mẫu này được cho vào mỗi chậu nhựa, nuôi trong nhiệt độ phòng để ấu trùng phát triển thành ấu trùng có sức gây nhiễm, ấu trùng giun lươn có sức gây bệnh được thu thập bằng phương pháp Baermann. Lợn gây nhiễm bằng 2 đường: đường tiêu hóa và đường qua da, với liều 10.000 ấu trùng/lợn. Kết quả nghiên cứu cho thấy lợn được gây nhiễm qua đường tiêu hóa cần khoảng 6 - 8 ngày để giun lươn hoàn thành vòng đời, trong khi gây nhiễm qua da cần khoảng 8 - 11 ngày để giun lươn hoàn thành vòng đời. Các triệu chứng lâm sàng ở lợn nhiễm giun lươn qua đường tiêu hóa, qua da và lợn nhiễm giun lươn ngoài thực địa đều có biểu hiện giống nhau như sốt, ho, kém ăn, con vật gầy, da khô, lông xù, niêm mạc nhợt nhạt và tiêu chảy.We carried out the experimental infection with strongyloidiasis for 15 one month old healthy pigs by dividing in three experimental batches (batch 1: strongyloides ransomi infected via pigs’ skin, batch 2: via oral and batch 3: control without infection), simuteanously observing clinical signs of 1,265 pigs infected naturally with strongyloides in each day in order to determine the time to complete the life cycle of Strongyloides ransomi and clinical characteristics of the infected pigs. The infection pathogens were reared as follows: Strongyloides ransomi eggs were collected from the feces and gastrointestinal contents of the infected pigs with very high intensity, these samples were placed in the plastic pots separately according to the sample type, raised at room temperature for larvae development, then they were collected by the Baermann method. The pigs were infected experimentally by 2 routes: oral and transdermal, with a dose of 10,000 larvae/pig. As a result, with the pigs infected via oral time for Strongyloides ransomi completing the life cycle was 6-8 days, while the period for Strongyloides ransomi completing its life cycle via transdermal infection required about 8-11 days. The clinical symptoms of pigs infected orally and trasndermally with strongyloides ransomi, and naturally infected pigs were similar, such as: fever, cough, poor appetite, thin animals, dry skin, ruffled fur, and mucous membranes. paleness and diarrhea.

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