Sự hiện diện của một số gen độc lực và gen kháng kháng sinh của vi khuẩn Escherichia Coli phân lập trên bò tại đồng bằng sông Cửu Long=Prevalence of virulence genes and antibiotic-resistance genes in Escherichia coli isolated from cattle in the Mekong delta

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Tác giả: Khánh Thuận Nguyễn, Lý Phương Vy Nguyễn, Trần Phước Chiến Nguyễn

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2024

Mô tả vật lý: tr.47

Bộ sưu tập: Metadata

ID: 486437

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Nghiên cứu này được thực hiện nhằm xác định sự hiện diện của một số gen độc lực và gen kháng kháng sinh trên 151 chủng vi khuẩn Escherichia coli phân lập từ bò ở đồng bằng sông Cửu Long trong năm 2022 bằng phương pháp PCR. Kết quả nghiên cứu cho thấy trong tổng số 151 chủng E. coli, có 74 chủng (49,01%) mang từ 1 đến 4 gen độc lực đã được xác định. Trong đó, các chủng chỉ mang gen stx1 hiện diện với tỷ lệ cao nhất (14,57%), kế đến là các chủng mang hai gen độc lực stx2 + hlyA (11,92%). Các chủng vi khuẩn E. coli đã được thử nghiệm về tính mẫn cảm của chúng với 13 loại kháng sinh bằng phương pháp khuyếch tán trên đĩa thạch. Kết quả nghiên cứu cho thấy các chủng E. coli nhạy cảm cao đối với hầu hết các loại kháng sinh, tuy nhiên vẫn có tỷ lệ cao các chủng E. coli đề kháng với ampicillin (47,68%), colistin (46,36%), streptomycin (43,71%), và tetracycline (41,72%). Các chủng E. coli này có thể đề kháng từ 1 đến 12 loại kháng sinh được khảo sát và đa kháng với 5 loại kháng sinh (18,54%) là phổ biến nhất. Kết quả kiểm tra sự hiện diện của 5 gen đề kháng kháng sinh (blaampC, blaTEM, blaCTX-M1, strA và aadA1) bằng phương pháp PCR cho thấy gen blaampC hiện diện trên tất cả 151 chủng E. coli (100%), kế đến là gen strA (45,70%), gen blaTEM (43,05%), và gen xuất hiện ít nhất là blaCTX-M1 (9,93%). Có 13 kiểu ghép gen kháng kháng sinh trên các chủng E. coli đã được ghi nhận, và kiểu ghép gen blaampC + blaTEM + strA xuất hiện phổ biến nhất (13,25%). Sự hiện diện của các gen độc lực và kháng kháng sinh trên vi khuẩn E. coli phân lập từ bò ở đồng bằng sông Cửu Long cho thấy mối nguy này cần được quan tâm nhằm bảo vệ sức khoẻ vật nuôi và sức khỏe cộng đồng ở đồng bằng sông Cửu Long.This study was conducted to determine the prevalence of virulence genes and antibiotic-resistance genes in 151 Escherichia coli strains isolated from cattle in the Mekong Delta in 2022, by PCR assay. The studied result showed that out of 151 isolated E. coli strains, there were 74 strains (49.01%) harboring from one to four virulence genes investigated. Among them, the rate of strains harboring the stx1 gene was the highest (14.57%), followed by the strains carrying two virulence genes: stx2 + hlyA (11.92%). Testing the antimicrobial susceptibility of the isolated E. coli strains with 13 antibiotics was carried out by Kirby–Bauer method. The tested result showed that the E. coli strains were sensitive with most of the antibiotics, However, there was still a high rate of E. coli strains resistant to ampicillin (47.68%), colistin (46.36%), streptomycin (43.71%), and tetracycline (41.72%). Those E. coli strains could resist from one to twelve antibiotics, and the resistance to 5 antibiotics (18.54%) was the most popular. The result of testing prevalence of five antibiotic-resistance genes (blaampC, blaTEM, blaCTX-M1, strA, and aadA1) showed that the blaampC gene was presented at all 151 E. coli strains (100%), followed by strA gene (45.70%), blaTEM gene (43.05%), and blaCTX-M1 (9.93%). Thirteen antibiotic-resistance patterns of E. coli strains were recognized, and the pattern of blaampC + blaTEM + strA was exhibited popularly (13.25%). The prevalence of virulence genes and antibiotic-resistance genes in E. coli isolated from cattle in the Mekong Delta indicate that these factors is a hazard that needs to concern to protect the animal and community health in the Mekong Delta.

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