Nghiên cứu tỷ lệ nhiễm, thành phần loài giun đũa (Toxocara spp.) ở chó tại Tỉnh Khánh Hòa=Study on prevalence, round worm species composition - Toxocara spp. in dogs in Khanh Hoa province

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Tác giả: Vũ Vỹ Huỳnh, Đức Quyết Lê, Hứa Ngọc Lực Lê, Đông Nguyễn, Đức Tân Nguyễn, Văn Thoại Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2024

Mô tả vật lý: tr.51

Bộ sưu tập: Metadata

ID: 486514

Thêm vào giỏ

A total of 337 fecal samples of dogs raising in Khanh Hoa province were collected and examined for Toxocara eggs by Füllerborn method. The examined results showed that the infection rate of dogs with Toxocara eggs was 28.48%, in which, the infection rate of dogs in Nha Trang city, Dien Khanh district and Khanh Vinh district was 18.58%, 24.13%, 43.51%, respectively. A total of 196 roundworm samples in dogs were collected and identified for species composition by morphology and PCR technique, ITS-2 gene sequencing. As a result, all the roundworm samples belonged to the Toxocara canis. The nucleotide sequences of Toxocara canis samples in dogs in Khanh Hoa province had a very high similarity level (99.4% -100%) and were similar to Toxocara canis strains on the GenBank (97% -100%). The Toxocara canis strains from different origins but they shared a very high similarity level and belonged to the same branch in the phylogenetic tree.Tổng số 337 mẫu phân chó nuôi tại tỉnh Khánh Hòa được thu thập và xét nghiệm bằng phương pháp phù nổi để xác định tỷ lệ nhiễm trứng giun đũa ở chó. Kết quả xét nghiệm cho thấy, tỷ lệ chó bị nhiễm trứng giun đũa là 28,48%
trong đó tỷ lệ nhiễm của chó ở thành phố Nha Trang, huyện Diên Khánh, huyện Khánh Vĩnh lần lượt là 18,58%
24,13% và 43,51%. Tổng số 196 mẫu giun đũa ở chó đã được xác định loài bằng hình thái học và kỹ thuật PCR, giải trình tự gen ITS-2. Kết quả là tất cả các mẫu giun đũa đều thuộc loài Toxocara canis. Trình tự nucleotide của các mẫu giun đũa ở chó tại tỉnh Khánh Hòa có tỷ lệ tương đồng rất cao (99,4%-100%) và tương đồng với các chủng Toxocara canis tham chiếu trên Ngân hàng Gen (97-100%). Các chủng Toxocara canis có nguồn gốc khác nhau nhưng chúng có mức tương đồng rất cao và cùng chung một nhánh trong cây phát sinh loài.

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