Thiết lập qui trình nhân giống in vitro cây mai vàng (Ochna integerrima (Lour.) Merr.)=Process of the micropropagation of Ochna integerrima (Lour.)Merr

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Tác giả: Hà Phạm Ngọc, Cẩm Nguyên Hồ Thị, Nhã Nguyễn Thị

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2019

Mô tả vật lý: tr.28

Bộ sưu tập: Metadata

ID: 487283

Thêm vào giỏ

Ở Việt Nam, mai vàng là một loại cây cảnh phổ biến, có giá trị kinh tế cao. Qui trình vi nhân giống mai vàng từ đoạn cành và búp chồi, được thiết lập qua 4 bước chính. Môi trường MS bổ sung cả BA và NAA với nồng độ tương ứng 1,5mg/lvà 0,5mg/l thích hợp để tái sinh chồi mai in vitro từ mẫu ban đầu. Môi trường MS bổ sung 2mg/l kinetin và 4mg/l BA được nghiên cứu thích hợp nhất để nhân số lượng chồi, đạt 3,4 chồi/mẫu, và có hiện tượng tạo cụm chồi từ mẫu búp chồi ban đầu. Nghiên cứu cũng cho thấy môi trường nền ½ MS thích hợp cho chồi mai vàng in vitro sinh trưởng, phát triển. Nồng độ GA3 0,5 - 1mg/l nên được sử dụng để kéo dài chồi trước khi đưa cây ra bầu trong giá thể gồm cát, đất, xơ dừa, trấu với tỉ lệ lần lượt là 30:50:10:10, cho tỉ lệ cây sống đến 70%.Ochna integerrima (Lour.) Merr. is a popular ornamental plant which has high economic value in Viet Nam. The process of in vitro propagation of Ochna integerrima (Lour.) Merr. using stem segments and shoot buds was established with four steps. On medium containing 1.5mg/l BA and 0.5mg/l NAA, shoots proliferated from stem segments have the best count and height of shoots. Medium contain 2mg/l kinetin and 4mg/l BA was the most suitable for propagation of Ochna integerrima's shoots (3.4 shoots/specimen) and found that clusters of shoot were created from shoot buds. The best medium for growing and developing of shoots was ½ MS. The concentration of 0.5 - 1mg/l GA3 should be added to the culture medium to extend the shoots. Then ex vitro Ochna integerrima (Lour.) Merr. were grown in a combination of soil, sand, coir and rice hush with the proportion of 30:50:10:10, achieving the survival rate of 70%.

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