Benzbromarone improves blood hypercoagulability after TBI by reducing phosphatidylserine externalization through inhibition of TMEM16F expression.

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Tác giả: Xin Chen, Yalong Gao, Kaiji Li, Lei Li, Tuo Li, Ruilong Peng, Cong Wang, Jinchao Wang, Guili Yang, Jianning Zhang, Shu Zhang

Ngôn ngữ: eng

Ký hiệu phân loại: 612.11 Blood

Thông tin xuất bản: Netherlands : Life sciences , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 487544

 AIMS: Traumatic brain injury-induced coagulopathy (TBI-IC) frequently occurs after TBI, exacerbating the severity of TBI and affecting patient prognosis. Benzbromarone (BBR) is commonly used to treat hyperuricemia
  however, its protective effects against TBI-IC remain unknown. Therefore, we explored whether BBR could improve TBI. MATERIALS AND METHODS: C57BL/6 wild-type mice were subjected to fluid percussion injury to mimic TBI, and BBR was administered intraperitoneally 30 min after TBI. Magnetic resonance imaging (MRI) and Evans blue dye extravasation were used to assess the prognosis, tail bleeding time, ELISA, and coagulation tests assess coagulation function. We further explored the potential mechanism by which BBR alleviates hypercoagulation after TBI using flow cytometry. KEY FINDINGS: The intraperitoneally injected BBR group showed improved survival and neurological severity scores compared to the TBI group. Subsequently, we found that hypercoagulability developed 3 h after TBI and that the administration of BBR improved this hypercoagulability. BBR also reduced the degree of platelet phosphatidylserine (PS) exposure after TBI, platelet activation, and Ca2+ overload, in addition to inhibition of scramblase activity in procoagulant platelets. SIGNIFICANCE: Our findings indicate that BBR reduces PS externalization by inhibiting TMEM16F expression, thereby improving blood hypercoagulability after TBI.
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