PHÂN LẬP VÀ ĐỊNH DANH CÁC CHỦNG VI KHUẨN CÓ KHẢ NĂNG TỔNG HỢP POLY-β-HYDROXYBUTYRATE (PHB) TỪ ĐẤT VÀ THỰC VẬT TẠI TỈNH BÌNH DƯƠNG=ISOLATION AND IDENTIFICATION OF POLY- β -HYDROXYBUTYRATE BIOSYNTHESIS FROM BOTANICAL RESOURCES IN BINH DUONG PROVINCE, VIETNAM

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Tác giả: Chánh Nguyễn Minh, Nguyễn Thành Luân, Nguyễn Thị Quỳnh Mai, Nguyễn Thị Liên Thương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2018

Mô tả vật lý: tr.12

Bộ sưu tập: Metadata

ID: 487600

Thêm vào giỏ

Bioplastic poly-β-hydroxybutyrate (PHB) derived from bacteria is known as asubstance with high thermal stability and ready biodegradability. The aim of this study was to isolate and identify some bacteria that would be able to synthesize plastic by biological pathway from soil and botanical resources in Binh Duong province, Vietnam. Optimization of incubation time periods was conducted to maximize PHB accumulation. The results of 16S rRNA sequencing indicated the bacterial isolates exhibited PHB synthesis are Rhizobium gallicum and Agrobacterium tuminfaciens. The highest PHB yield was 39,84% obtained when Rhizobium gallicum was cultured in 48 hours period. The FTIR spectrum ofbioplastic sample showed the similarity in its characteristics to the commercial PHB. The present study has thus provided useful data on some bacterial isolates for PHB production that can be potential for industrial application in manufacture of PHB, an emerging alternative of non-biodegradable plastics.Keywords: Poly-β–hydroxybutyrate, PHB, Rhizobium gallicum, Agrobacterium tuminfaciens, bioplasticNhựa sinh học có nguồn gốc chủ yếu từ vi khuẩn, có khả năng chịu nhiệt tốt và có thể phân hủy sinh học nhanh. Nghiên cứu này phân lập và tuyển chọn các nguồn vi khuẩn có khả năng sinh tổng hợp nhựa sinh học từ đất và thực vật tại tỉnh Bình Dương. Khi khảo sát hàm lượng và thời gian thu nhận nhựa PHB ở 3 chủng CR2, RB3, RC3 cho thấy chủng vi khuẩn CR2 hoang dại có khả năng sinh tổng hợp PHB cao nhất đạt 39,84% trong thời gian 48 giờ và đạt giá trị màu sắc tốt nhất khi hòa tan bằng H2SO4 đậm đặc. Kết quả định danh cho thấy chủng CR2 là loài Rhizobium gallicum, RB3 là loài Agrobacterium tuminfaciens. Cấu trúc PHB thu nhận được giống với cấu trúc của PHB thương phẩm. Nghiên cứu chuyên sâu về PHB là tiềm năng phát triển cho một nền công nghiệp và nông nghiệp xanh, cải tiến các phương pháp sản xuất nhựa PHB ở quy mô công nghiệp trong vật liệu mới thay thế.Từ khóa: Poly-β-hydroxybutyrate, PHB, Rhizobium gallicum, Agrobacterium tuminfaciens, nhựa sinh học.

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