NÂNG CAO SỰ TÍCH LUỸ ASTAXANTHIN Ở VI TẢO Haematococcus pluvialis BỞI CÁC ĐIỀU KIỆN STRESS CỦA MÔI TRƯỜNG NUÔI CẤY=ENHANCEMENT OF ASTAXANTHIN ACCUMULATION IN Haematococcus pluvialis UNDER STRESS CONDITIONS

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Tác giả: Hiền Nguyễn Thị Duy, Hiếu Huỳnh Thị, Trần Thị Bích Liên, Nam Trịnh Ngọc, Trương Ngọc Bảo Trân

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2017

Mô tả vật lý: tr.48

Bộ sưu tập: Metadata

ID: 487799

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The unicellular microalgae (Haematococcus pluvialis) is one of the potential organisms with ability to produce astaxanthin. Astaxanthin is a high value ketocarotenoid, which is widely used as antioxidant in food, pharmaceutical and natural colorant in aquaculture. H. pluvialis accumulates large amounts of astaxanthin under stress conditions. This study was conducted to determine the optimal medium for biomass production and factors that influenced the astaxanthin accumulation by H. pluvialis. Investigation of media including Bold’s Basal, OHM (optimal Haematococcus medium), RM (Rudic medium), f/2 Guillard and Walne showed that H. pluvialis grew well in RM medium after 18 days. The maximum cell density of 6.97 x 105 cells/mL was achieved in RM medium. Nitrogen starvation by removing nitrate or reducing nitrate source in the culture medium induced astaxanthin production. Under high light conditions (4 klux and 8 klux) and high concentration of carbon dioxide, an increase in astaxanthin accumulation was observed. Analyses using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) confirmed the presence of astaxanthin in H. pluvialis cell extract. Furthermore, liquefied dimethyl ether (DME) can be used to extract astaxanthin directly from H. pluvialis biomass with high moisture content.Keywords: astaxanthin, bioreactor, dimethyl ether, high-performance liquid chromatography, microalgae Haematococcus pluvialisVi tảo đơn bào Haematococcus pluvialis là một trong những vi tảo có tiềm năng trong sản xuất astaxanthin, một loại ketocarotenoid, được sử dụng rộng rãi như một chất kháng oxy hoá trong thực phẩm, dược phẩm và chất tạo màu tự nhiên trong nuôi trồng thủy sản. Sự tích lũy astaxanthin trong tế bào H. pluvialis thường xảy ra trong điều kiện stress. Trong nghiên cứu này, môi trường phù hợp cho sự sinh trưởng và các yếu tố ảnh hưởng đến sự tích tụ astaxanthin của vi tảo H. pluvialis được xác định. So sánh giữa các môi trường khảo sát gồm Bold’s Basal, OHM (optimal Haematococcus medium), RM (Rudic’s medium), f/2Guillard và Walne, kết quả cho thấy trên môi trường RM, sự sinh trưởng của tảo thuận lợi nhất. Mật độ cực đại đạt được sau 18 ngày nuôi cấy ở mức 6,97 x 105 tế bào/mL. Sự thiếu hụt nguồn dinh dưỡng nitơ do sự loại bỏ hoàn toàn hoặc một nửa lượng nitrate trong môi trường nuôi cấy đã cảm ứng sự sản sinh astaxanthin trong tế bào tảo. Điều kiện cường độ chiếu sáng mạnh (4 klux và 8 klux) và nồng độ CO2 cao cũng đã kích thích sự tích lũy astaxanthin. Sử dụng phương pháp sắc ký bản mỏng (thin-layer chromatography - TLC) và phân tích sắc ký lỏng hiệu năng cao (high-performance liquid chromatography - HPLC) đã xác nhận sự hiện diện của astaxanthin trong dịch chiết tế bào tảo H. pluvialis. Ngoài ra, trong nghiên cứu này, astaxanthin trong sinh khối ướt của tảo đã được tách chiết bằng dung môi an toàn dimethyl ether định hướng ứng dụng cho thực phẩm.Từ khóa: Astaxanthin, bioreactor, dimethyl ether, sắc ký lỏng hiệu năng cao, vi tảo Haematococcus pluvialis.

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