Deoxynivalenol (DON) is a common contamination mycotoxin that which exerts significant hepatotoxicity, posing a serious threat to human and animal health. Ferroptosis has been linked to the development of hepatotoxicity induced by DON. However, the mechanism by which DON promotes ferroptosis in hepatocytes is not well understood. Although studies have shown that DON upregulates the expression of nicotinamide N-Methyltransferase (NNMT), its role in DON hepatotoxicity has not been elucidated. In this study, we found that DON inhibited SLC7A11/GPX4 and increased cytosolic free Fe