Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b.

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Tác giả: Eun Yeol Lee, Ok Kyung Lee, Jeong-Geol Na, Diep Thi Ngoc Nguyen

Ngôn ngữ: eng

Ký hiệu phân loại: 612.0154 Human physiology

Thông tin xuất bản: Netherlands : New biotechnology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 487909

 We report a biosynthetic method for producing poly(3-hydroxybutyrate-co-3-hydroxypropionate) [P(3HB-co-3HP)] copolymer from methane alone
  we developed a 3-hydroxypropionate (3HP) biosynthetic pathway starting from methane in Methylosinus trichosporium OB3b and overexpressed 3HP-CoA transferase to optimize 3HP incorporation into P(3HB-co-3HP) copolymers. Upon comparing the β-alanine and malonyl-CoA pathways, we discovered that the latter showed greater potential for the formation of 3HP monomers. In addition, we examined the activities of 3HP-CoA synthetases from three distinct sources and found that the enzyme from Metallosphaera sedula was the most effective for 3HP incorporation. Using methane as the sole carbon source in a shake-flask culture, we developed recombinant strains and found that the best strain was OB3b-MCRM-3S, which formed a P(3HB-co-3HP) copolymer, up to 25.62% of the biomass, with a maximum 3HP level of 9.02mol%. Our research demonstrates the successful production of a biopolymer, the P(3HB-co-3HP) copolymer, via methane bioconversion using methanotrophs.
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