The action of microorganisms can lead to a decrease in food freshness and cause food safety issues. Traditional packaging can only provide a certain antimicrobial effect and cannot indicate the freshness of food, which can no longer meet the needs of consumers. The preparation of intelligent packaging films with antimicrobial effects and freshness indication is an effective solution. In this study, A novel electrospun intelligent food packaging film based on polylactic acid/eugenol/coumarin derivatives (7-(diethylaMino)-2-oxo-2H-chroMene-3-carbaldehyde) has been constructed to achieve the dual functions of antimicrobial and freshness indication for chilled pork. The antimicrobial layer loads eugenol coaxially into the fiber, which could scavenge 82.92 % of DPPH free radicals and effectively inhibit the growth of Escherichia coli and Staphylococcus aureus. The freshness-indicating layer showed fluorescence responsiveness to biogenic amines. The freshness-indicating and antimicrobial fibrous bilayer film turned green in practical application to indicate that the refrigerated pork had spoiled and was effective in extending the shelf life by 4-6 days. This study demonstrated the effectiveness of the bilayer film for preservation and freshness non-destructive testing of chilled pork. It provided a theoretical basis for the general application of film.