The oil palm is a prominent tropical oil crop with holds considerable economic value. MYB transcription factors are key regulators in growth and plant stress adaptation mechanisms in plants. However, the roles and operational mechanisms of MYB genes in oil palm are not yet well understood. In this study, EgMYB111 was cloned from oil palm, and its behavior under cold stress was examined in genetically engineered tobacco and oil palm embryoids. Physiological and biochemical analysis demonstrated that genetically engineered lines exhibited substantially greater cold tolerance than control plants. EgMYB111 was noticed to localize within the nucleus, and cold stress significantly enhanced the expression of the GUS gene managed by the EgMYB111 expression regulator. Interestingly, EgMYB111 was involved in the reaction to stress via an abscisic acid (ABA)-mediated pathway. Yeast two-hybrid experiments confirmed the involvement of EgMYB111 and the ABA receptor proteins PYR1 and PYL9. Moreover, the transient transformation of oil palm protoplasts combined with qRT-PCR analysis revealed that the over-activity of EgMYB111 induced a significant induction of the genes EgSnRK2.1, EgSnRK2.3, and EgSnRK2.5. In addition, dual-luciferase analyses, yeast one-hybrid assays, and electrophoretic mobility shift assays (EMSA) established that EgMYB111 binds to the promoters of EgSnRK2.1, EgSnRK2.3, and EgSnRK2.5, thereby regulating their transcription and enhancing low-temperature resilience in oil palm. The work concludes that the EgMYB111 performs a key role in augmenting cold adaptability in oil palm by governing the transcription of key genes utilizing an ABA-regulated pathway.