BACKGROUND: The pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) remains a subject of discussion. Although both microbial infection and autoimmunity have been proposed as potential contributors to CRSwNP pathobiology, their respective roles and intricate interactions in disease progression remain unclear owing to the limited knowledge regarding dysregulated humoral immunity in CRSwNP. OBJECTIVE: To deepen our understanding of CRSwNP, we sought to identify the precise humoral antigens targeted by dominant B-cell clones within the disease environments. METHODS: Immunoglobulin repertoire sequencing was performed to identify dominant B-cell clones in CRSwNP tissues. These immunoglobulin clones were reconstructed as antibodies, which were then used in immunoprecipitation and antigen array experiments for hypothesis-free global antigen profiling of auto- and exogenous antigens. RESULTS: From the analysis of 13 CRSwNP patients, 31 antibodies were reconstructed from dominant B-cell clones identified in nine patients. Seven novel protein autoantigens were identified, five of which were nucleic acid-binding proteins, and all were associated with autoimmune diseases. Additionally, nine microbial antigens, including various viruses, bacteria, and fungi, were discovered. Notably, two antibodies demonstrated dual reactivity, simultaneously recognizing both microbial and human proteins. For example, one antibody targeted CMV, Clostridium tetani, and human plectin (PLEC), while another recognized Aspergillus niger and human dihydrolipoamide S-acetyltransferase (DLAT), through molecular mimicry of shared amino acid homologies. CONCLUSION: Our data indicate the possibility that the pathobiology of CRSwNP involves autoreactive humoral immunity, with a subset of cases potentially exhibiting molecular mimicry-driven autoimmune features triggered by microbial infections. Nevertheless, this hypothesis requires further investigation.