ĐỊNH LƯỢNG ĐỒNG THỜI CAFEIN, THEOBROMIN VÀ THEOPHYLLIN TRONG CHÈ BẰNG PHƯƠNG PHÁP QUANG PHỔ HẤP THỤ PHÂN TỬ (UV/VIS) KẾT HỢP VỚI HỒI QUI ĐA BIẾN = SIMULTANEOUS DETERMINATION OF CAFFEINE, THEOBROMINE, THEOPHYLLINE IN TEA USING ULTRAVIOLET - VISIBLE SPECTROSCOPY COMBINED WITH MULTIVARIATE ANALYSIS

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Tác giả: Huế Trần Thị, Thảo Tạ Thị, Thùy Dung Trần Thị, Ri Nguyễn Văn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Phân tích Hóa, Lý và Sinh học, 2019

Mô tả vật lý: tr.192

Bộ sưu tập: NCBI

ID: 488705

An analytical procedure based on the use of ultraviolet – visible spectroscopy combined with multivarite regression was applied for simultaneous determination of caffeine, theobromine and theophylline in 53 Vietnamese tea samples. The 240-310nm spectral window was used for data acquisition.The partial least squares (PLS) and Artificical Neural Network (ANN) method were used to calculate concentration of 3 ananlytes in samples. The same model of PLS and ANN with standard matrix of 44 tea samples (the contents of 3 analytes were determined by high performance liquid chromatography) were applied for individual and/or simultaneous determination of caffeine, theobromine and theophylline in samples. A matrix of test samples including 9 tea samples was used to assess the accuracy of the models. Compared with PLS, the ANN model was better with lower realtive error. This work demonstrated that ultraviolet – visible spectrophotometry with ANN model could be simply used to analyse xanthine’s contents in tea with simple sample preparation.Keywords: caffeine, theobromine, theophylline, multivariable calibration, tea, ultraviolet – visible spectrophotometry
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