KHẢO SÁT QUÁ TRÌNH TẠO CHẾ PHẨM BROMELAIN DẠNG BỘT TỪ PHU PHẨM DỨA=STUDY ON PRODUCTION OF BROMELAIN POWDER FROM PINEAPPLE WASTES

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Tác giả: Đào Thị Mỹ Linh, Nguyễn Thị Quỳnh Mai, Nguyễn Thị Như Phượng, Tuyến Đỗ Thị Hoàng

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2017

Mô tả vật lý: tr.19

Bộ sưu tập: Metadata

ID: 488792

Thêm vào giỏ

Nghiên cứu này được thực hiện nhằm thu chế phẩm bromelain dạng bột từ phụ phẩm dứa bằng phương pháp sấy phun. Kết quả cho thấy, chất trợ sấy phù hợp là sữa tách béo (skim milk) với nồng độ 10% (w/v), quá trình sấy thực hiện ở nhiệt độ không khí đầu vào 110 oC, nhiệt độ không khí đầu ra 60 oC, tốc độ bơm nhập liệu 288 mL/h (8 rpm). Tạo được chế phẩm bromelain co hoạt tính 1542,550 ± 98,384 (UI/g), thông số động hoc Km = 2,031 mM và Vmax = 0,054 mM/phút với cơ chất là casein, hoạt động thủy phân tốt nhất ở pH 7, nhiệt độ 40 oC. Chế phẩm thích hợp bảo quản ở 0 oC trong bao nhôm và có thể giữ được 50% hoạttính sau 20 ngày.Từ khóa: Enzyme, bromelain, loc tiếp tuyến, phụ phẩm dứa, sấy phunThis study was conducted to produce bromelain powders from pineapple wastes by spray drying. The results showed that the suitable carrier agent was skim milk at a concentration of 10% (w/v). To obtain the highest activity of bromelain enzyme, the drying process was carried out at inlet air temperature of 110 °C, outlet air temperature of 60 °C, feed flow rate of 288 mL/h (8 rpm). The obtained bromelain powder had activity of 1542,550 ± 98,384 (UI/g), dynamic parameters Km = 2,031 mM and Vmax = 0,054 mM/min with casein substrate, hydrolysis activity at pH 7 and 40 oC. Bromelain powder was stored at 0 °C in aluminum bags and can be kept 50% activity after 20 days.Keywords: Enzyme, bromelain, membrane processing, pineapple wastes, spray drying

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