NGHIÊN CỨU CHỌN NHUYỄN THỂ HAI MẢNH VỎ CHỨA PROTEASE HOẠT TÍNH CAO VÀ XÁC ĐỊNH MỘT SỐ ĐẶC TÍNH CỦA ENZYME=SELECTION OF BIVALVE SPECIES CONTAINING HIGHLY-ACTIVE PROTEASE AND DETERMINATION OF SOME PROPERTIES OF ENZYME

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Tác giả: Trần Quốc Đảm, Đào Thị Tuyết Mai

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2017

Mô tả vật lý: tr.118

Bộ sưu tập: Metadata

ID: 488810

Thêm vào giỏ

Protease có khả năng thủy phân protein thành các peptid và acid amin hay còn gọi là dịch đạm thủy phân đươc ứng dụng trong nhiều ngành công nghiệp. Trong nghiên cứu này, chúng tôi tiến hành tách chiết protease từ một số loại nhuyễn thể hai mảnh vỏ và khảo sát một số đặc tính của enzyme này. Tiến hành đánh giá hoạt độ protease từ một số nhuyễn thể hai mảnh vỏ bằng phương pháp Anson cải tiến. Kết quả lựa chọn đươc nhuyễn thể sò lụa chứa hệ protease có hoạt tính cao. Xác định đươc điều kiện tách chiết protease thích hơp từ nội tạng sò lụa là: đệm phosphat với tỷ lệ mẫu/dung môi là 1/2, nhiệt độ chiết là 35 oC và thời gian chiết là 10 phút. Protease thu đươc hoạt động thích hơp ơ nhiệt độ 50 oC, pH 5,5 và có khả năng hoạt động thủy phân trong khoảng thời gian 5 giờ.Từ khóa: Protease, nhuyễn thể hai mảnh vỏ, sò lụa, sò điệp, sò lôngProtease is able to hydrolyze the proteins into peptides and amino acids or known as hydrolyzed protein which is popularly used in many industries. In this study, we extracted the protease from some bivalve species and determined some properties of this enzyme. Evaluation of proteolytic activity from some bivalve species was carried out using the modified Anson’s method. The results showed the short-neck clam (Paphia undulata) contained highly active proteases. The optimum conditions for extraction of protease from the short-neck clam were determined as follows: ratio of sample to phosphate buffer at 1/2
extraction temperature at 35 °C and extraction duration of 10 minutes. This protease activity is the best at 50 oC, pH 5.5 and hydrolysis for 5 hours.Keywords: Protease, bivalve, Paphia undulata, Mimachlamys nobilis, Anadara subcrenata

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