NGHIÊN CỨU TƯƠNG TÁC VÀ KHAI THÁC DỮ LIỆU BIỂU HIỆN GIỮA CÁC TIỂU PHẦN CỦA YẾU TỐ NHÂN Y Ở LOÀI ĐẬU GÀ (Cicer arietinum) = INTERACTION AND DATA MINING OF EXPRESSION OF THE NUCLEAR FACTOR-Y SUBUNITS IN CHICKPEA (Cicer arietinum)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Chu Đức Hà, QuỳNh Lê Thị NgọC

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý: tr.45977

Bộ sưu tập: NCBI

ID: 488867

Thêm vào giỏ

Nghiên cứu về tương tác protein và mức độ biểu hiện của gen mã hóa protein đích được xem là những công cụ hỗ trợ đắc lực cho việc đưa ra giả thuyết về chức năng của gen. Trong nghiên cứu này, khả năng tương tác giữa ba tiểu phần của nhân tố phiên mã Yếu tố nhân Y (NF-Y) và mức độ biểu hiện của các gen mã hóa đã được phân tích trên cây đậu gà (Cicer arietinum). Kết quả mô hình hóa interactome đã cho thấy tiểu phần CaNF-YB có thể kết hợp chặt chẽ với CaNF-YC thành dạng nhị hợp, sau đó tương tác với CaNF-YA tạo thành phức hợp CaNF-Y hoàn chỉnh, tương tự như ở các loài thực vật khác. Trong đó, bốn dạng kết hợp của ba tiểu phần đã được dự đoán là [(CaNF-YB19/-YC01)/-YA06], [(CaNF-YB19/-YC01)/-YA02], [(CaNF-YB18/-YC01)/-YA06] và [(CaNF-YB18/-YC01)/-YA02]. Tiếp theo, cấu trúc vùng bảo thủ của các tiểu phần CaNF-YA có tương tác đều chứa hai vùng riêng biệt, là đoạn nhận biết, bám DNA và đoạn tương tác với cấu trúc CaNF-YB/YC. Cấu trúc vùng bảo thủ của các tiểu phần CaNF-YB và -YC có tương tác được ghi nhận với hai vùng tương tác với CaNF-YA và với CaNF-YC hoặc CaNF-YB. Khai thác dữ liệu phiên mã trước đây đã chỉ ra rằng các gen mã hóa tiểu phần CaNF-Y có tương tác đều thể hiện mức độ biểu hiện mạnh ở ít nhất một vị trí trong cây. Kết quả của nghiên cứu này đã gợi mở những dẫn liệu quan trọng cho việc định hướng nghiên cứu chức năng của các gen mã hóa tiểu phần CaNF-Y quan tâm. Protein - protein interaction and data mining of the expression patterns of genes were considered as the major reliable tools to construct the theory of the gene functions. Here, the interactions between Nuclear factor - Y (NF-Y) subunits and the expression profiles of corresponding genes found in chickpea (Cicer arietinum) were performed. Based on the interactome database, we predicted that members of CaNF-YB subunits can slightly interact with members of CaNF-YC subunit to construct the heterodimer, which then merges with CaNF-YA subunit to create the CaNF-Y complex as fully reported in other plant species. Among them, four formulas of protein-protein interaction, including [(CaNF-YB19/-YC01)/-YA06], [(CaNF-YB19/-YC01)/-YA02], [(CaNF-YB18/-YC01)/-YA06] and [(CaNF-YB18/-YC01)/-YA02] were predicted. Next, the conserved domain of interacted CaNF-YA subunit consisted of two distinct sub-domains, including the DNA binding sub-domain and the CaNF-YB/YC interaction domain. The conserved domains of interacted CaNF-YB and -YC subunits were reported to harbor two regions, CaNF-YA interaction sub-domain and CaNF-YC or -YB interaction sub-domain. Of our interest, data mining of the previous transcriptome atlas revealed that genes encoding interacted CaNF-Y subunits were highly expressed in at least one organ. Taken together, out study would provide the important information for the further characterization of genes encoding CaNF-Y subunits.

Tạo bộ sưu tập với mã QR