Nghiên cứu tác động của nano kẽm oxide và nano cobalt đối với quá trình nảy mầm ở hạt đậu tương (glycine max (l.) merr.)

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Tác giả: Quỳnh Liên Lê, Thị Thu Hiền Lê, Quốc Bưu Ngô, Chi Mai Nguyễn, Hoài Châu Nguyễn, Tường Vân Nguyễn, Khắc Bản Ninh, Thị Hòe Phạm, Mỹ Linh Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Công nghệ sinh học, 2018

Mô tả vật lý: 501-508

Bộ sưu tập: Metadata

ID: 489187

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Chủ đề

Ảnh hưởng của nano kẽm oxide (ZnO) và nano cobalt (Co) đối với quá trình nảy mầm của hạt đậu tương (Glycine max (L.) Merr) được đánh giá thông qua các chỉ tiêu về tốc độ nảy mầm, sự phát triển rễ mầm và mức độ biểu hiện của một số gen quan trọng. Kết quả cho thấy, xử lý hạt giống đậu tương ĐT26 với các dung dịch nano kim loại đã thúc đẩy tốc độ nảy mầm và tăng cường sự phát triển rễ mầm, đồng thời không làm ảnh hưởng tới sự phát sinh hình thái của cây mầm. Trong các nồng độ nano thử nghiệm, nano ZnO 50 mg/L và nano Co 0,05 mg/L cho tác động tốt nhất tới quá trình nảy mầm và sự phát triển rễ mầm.Nowaday, nanotechnology and nanomaterials have been applied widely as stimulars and micronutrions in agricultural production to increase productivity and quality of important crops. In order to promote the efficiency and stable use of nano metal products for such purpose in Vietnam, several studies have focused on evaluating new metal nanoparticles in different plant species. In this study, the effects of zinc oxide (ZnO) and cobalt (Co) nanoparticles on germination of soybean (Glycine max (L.) Merr), an important crop in Vietnam, were assessed through germination rate, root growth and the expression of important genes. The results showed that DT26 soybean seeds treated with metal nanoparticles at extremely low concentrations accelerated the germination rate and enhanced root growth, however did not affect morphogenesis. Nanoparticles of ZnO of 50 mg/L and Co of 0.05 mg/L provided the highest effect on both germination and root growth. Expression analysis of genes encoding key enzymes in germination processs of soybean such as(:) Aminocyclopropane-1- carboxylic acid synthase 2 (ACS2), Lypoxygenase 3 (LOX9-03), Lypoxygenase 7 (LOX9-07), Amylase 8 (AMY8), Saccharogen amylase (AMYS), Alpha-glucan dikinase (α-GLU), Urease 2 (URE02), Urease 14 (URE14) indicated that the expression of most studied genes were increased significantly in primary roots of nano treated seeds compared to the control. The obtained results can be a scientific evident to support the application of nano products in agricultural practice.

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