ẢNH HƯỞNG CỦA CHẤT ĐIỂU HÒA TĂNG TRƯỞNG THỰC VẬT ĐẾN KHẢ NĂNG TẠO MÔ SẸO TỪ THÂN VÀ LÁ IN-VITRO CỦA CÂY OẢI HƯƠNG (Lavandula dentata) = EFFECT OF PHYTOHORMONES ON IN-VITRO CALLUS FORMATION FROM LEAVES AND STEMS OF LAVENDER (Lavandula dentata)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Trịnh Thị Hương, Nhung Nguyễn Thị Tuyết, Thoa Trần Thị Anh, Lê Thị Thúy

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2019

Mô tả vật lý: tr.19

Bộ sưu tập: NCBI

ID: 489218

Thêm vào giỏ

Formations and morphogenesis in callus were investigated in Lavandula dentata. Callus was obtained from different parts of plants such as leaves and stems cultured on MS medium (Murashige & Skoog) supplemented with NAA (Naphthylacetic acid) and BA (Benzyl adenine). After 60 days of in-vitro culture, leaves culturing in MS medium with NAA 0.1 mg/L and BA 2.0 mg/L was most suitable for callus formation leading to shoot buds multiplication due to these calluses. On the other hand, MS medium supplemented with NAA 1.0 mg/L and BA 2.0 mg/L was also used to give callus which was 100% callus formation. Stem in-vitro was used as callus sources to culture on MS medium having phytohormones, the best result was 100% callus formation in MS medium with NAA 2.0 mg/L and BA 1.0 mg/L which allowed shoot buds to multiply. Keywords: BA, NAA, callus, in-vitro, Lavender (Lavandula dentata). Khả năng tạo mô sẹo và hình thái mô sẹo được tiến hành nghiên cứu trên cây oải hương Lavandula dentata. Mô sẹo được thu nhận từ lá và thân in-vitro trên môi trường MS (Murashige & Skoog, 1962) có bổ sung NAA (Naphthylacetic acid) và BA (Benzyl adenine). Sau 60 ngày nuôi cấy, mẫu lá nuôi trên môi trường MS bổ sung NAA 0,1 mg/L và BA 2,0 mg/L có khả năng tạo sẹo và phát sinh chồi. Với mẫu lá in-vitro nuôi cấy trên môi trường MS bổ sung NAA 1,0 mg/L và BA 2,0 mg/L cũng thu nhận được mô sẹo với tỷ lệ 100%. Thân in-vitro tạo được mô sẹo trên môi trường MS có bổ sung NAA 2,0 mg/L và BA 1,0 mg/L với tỷ lệ 100% thích hợp cho nguồn mẫu vi nhân giống in vitro cây oải hương Lavandula dentata. Từ khóa: Benzyl adenine, Naphthylacetic acid, mô sẹo, in-vitro, cây oải hương, Lavandula dentata.

Tạo bộ sưu tập với mã QR