Hoạt tính kháng oxy hóa và đặc điểm ức chế enzym acetylcholinesterase của cao chiết methanol lá cây lá đắng (Vernonia amygdalina DeL. ), họ cúc (Asteraceae)

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Tác giả: Đình Chung Dương, Hữu Khánh Quan Nguyễn, Thị Cẩm Duyên Nguyễn, Thị Ngọc Yến Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 571.952 Physiology and related subjects

Thông tin xuất bản: Tạp chí Công Thương, 2018

Mô tả vật lý: 500-507

Bộ sưu tập: Metadata

ID: 490291

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Alzheimer's disease is an age-related neurological disease characterized by high oxidative stress and acetylcholine (AChE) levels in the brain. Vernonia amygđalyna has a number of beneficial pharmacological effects such as antioxidant, anti-inflammatory and memory enhancement activities. From the methanol extract of Vernonia amygdalina leaves the different segments in the high were evaluated for antioxidant activity and AChE resistance. Vernonia amygdalina leaves are extracted with methanol and fractional with n-hexane, ethyl acetate, chloroform and butanol solvents. These segments were investigated for antioxidant activity by DPPH test and acetylcholinesterase inhibitory activity by Ellman's colorimetric method. The fraction of butanol extract had the strongest antioxidant activity with IC50 = 41.43 ± 0.36 pg / mL. The strongest inhibitory activity of AChE is ethyl acetate extract with an IC50 value of 59.58 ± 0.99 pg / mL in a concentration-dependent manner. The kinetic inhibition analysis showed that the ethyl acetate fraction of the mixed inhibitor with Ki (representing enzyme and inhibitor affinity) was 0.53 ± 0.03 mg / mL. The result is a prerequisite for further research on the biological activity of Vernonia amygdalina in search of new materials used in the prevention and treatment of neurological disorders such as Alzheimer's.Bệnh Alzheimer là một bệnh về thần kinh có liên quan đến tuổi tác, bệnh được đặc trưng bởi sự stress oxy hóa cao và nồng độ acetylcholine (AChE) trong não. Vernonia amygđalyna có một số tác dụng dược lý có lợi như các hoạt động chống oxy hóa, chống viêm và tăng cường trí nhớ. Từ cao chiết methanol của lá Vernonia amygdalina các phân đoạn khác nhau trong cao được đánh giá hoạt tính chống oxy hóa và kháng AChE. Lá của Vernonia amygdalina được chiết xuất bằng methanol và tách phân đoạn với các dung môi n-hexan, ethyl acetat, chloroform và butanol. Những phân đoạn này được nghiên cứu hoạt tính chống oxy hóa bằng thử nghiệm DPPH và hoạt tính ức chế acetylcholinesterase bằng phương pháp đo màu của Ellman. Kết quả phân đoạn dịch chiết butanol có hoạt tính chống oxy hóa mạnh nhất với IC50 = 41,43 ± 0,36 pg/mL. Hoạt tính ức chế AChE mạnh nhất là của dịch chiết ethyl acetat với giá trị IC50 = 59,58 ± 0,99 pg/mL theo cách phụ thuộc nồng độ. Phân tích ức chế động học cho thấy phần ethyl acetat của loại ức chế hỗn hợp với Ki (đại diện cho ái lực của enzym và chất ức chế) là 0,53 ± 0,03 mg/mL. Kết quả là tiền đề cho những nghiên cứu sâu hơn về hoạt tính sinh học của Vernonia amygdalina nhằm tìm kiếm các nguyên liệu mới được sử dụng trong phòng bệnh, điều trị các rối loạn thần kinh như Alzheimer.

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