Nhận diện và đánh giá tính chống chịu mặc của các giống lúa mùa dựa trên dấu phân tử SSR (Simple Sequence Repeats)

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Tác giả: Lam Minh Nguyễn, Văn Mịch Phạm, Hữu Phúc Trần, Thị Xuân Mai Trần, Anh Pháp Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 571.962 Physiology and related subjects

Thông tin xuất bản: Khoa học (Đại học Cần Thơ), 2018

Mô tả vật lý: 82-89

Bộ sưu tập: Metadata

ID: 491974

Thêm vào giỏ

Để nhận diên dấu phân tử liên kết chặt với gen chịu mặn các giống lúa mùa,cả kiểu hình và kiểu gen chịu mặn giai đoạn mạ, nhóm nghiên cứu đã thanh lọc tính chịu mặn ở mức EC = 12 mS/cm theo quy trình của Viện Nghiên cứu Lúa quốc tế. Kết quả thí nghiệm có 23,7% giống lúa được đánh giá là chịu mặn, 28,9% chịu mặn trung bình 39,5% nhiễm mặn và 7,9% rất nhiễm mặn. Về kiểu gen, 2 dấu phân tử các chuỗi lặp lại đơn (simple sequence repeats - SSR) là RM493 và RM3412 được chọn để nhận diện kiểu gen chịu mặn. Hiệu quả đánh giá bằng RM493 và RM3412 đạt từ 70% trở lên ở tất cả các nhóm cấp độ chịu mặn. Dựa trên hai dấu SSR này, 36 giống lúa mùa được chia thành 7 nhóm chính theo phương pháp UPGMA. Nhóm I có các giống có khả năng chịu mặn khá. Các giống có kiểu gen chịu mặn trung bình hầu hết thuộc nhóm II và III. Các kiểu gen nhiễm và rất nhiễm mặn thuộc các nhóm từ IV đến VII. Kết quả RM493 và RM3412 là dấu phân tử hữu ích trong chọn lọc các giống lúa mùa chịu mặn.To identify molecular markers linked to salt-tolerant gene of rice, both phenotypic and genotypic evaluation of salt tolerance were performed atseedling stage. The phenotypic screening forsalinity stress at EC=12 mS/cm was doneusing the standard protocol of the International Rice Research Institute. About 23.7% of rice varieties were identified as salt tolerant, 28.9% were moderately tolerant, 39.5% were susceptible and 7.9% were highly susceptible. Two SSR markers (RM493 and RM3412) have shown the ability (over 70%) for salt tolerant identification in all groups. Additionally, 36 rice varieties were grouped into 7 major clusters by UPGMA method based on these markers. Most of tolerant genotypes belonged to cluster I while moderately tolerant genotypes were in cluster II and III. From clusters IV to VII included susceptible and highly susceptible genotypes. These results indicated that RM493 and RM3412 were useful for marker assisted selection at seedling stage.

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