Ảnh hưởng của chế độ chiếu sáng bằng đèn LED lên khả năng sinh trưởng và phát triển của chồi hai loài kim tuyến (Anoectochilus annamensis Aver. và Anoectochilus roxburghii (Wall.) Wall. ex Lindl.) nuôi cấy In vitro

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Tác giả: Thị Thanh Phương Bùi, Thị Phương Lan Nguyễn, Hương Sơn Phạm, Xuân Bình Minh Phan, Minh Hợi Trần, Thị Thảo Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 581.3 +Genetics and evolution

Thông tin xuất bản: Sinh học, 2018

Mô tả vật lý: 32-38

Bộ sưu tập: Metadata

ID: 492064

Thêm vào giỏ

Anoectochilus annamensis and A. roxburghii belong to Orchidaceae family that has medicinal and ornamental plant value. They are endangered plants in the wild due to the over- collected and loss of the, suitable habitats. Using the LED lighting source for culture these species in vitro condition to optimize the culture conditions, reduction of the production cost, especially electric bill for air-conditionning, lighting. In recent years, the trial applied LED which has the feature of energy saving, small size and a longer operating life, for plant production has started. In this study, LED illumination sources are in four different wavelengths of λ= 430- 470 nm
λ= 470-510 nm
λ= 510-560 nm
λ= 560-600 nm and white fluorescent lamp as control with light intensity photosynthetic photon flux density (PPFD) of 40 μmol/m2/s photon used to study their effects on the growth and development of A. annamensis and A. roxburghii species. After 8 weeks of implementing, the results showed that the LEDs of λ= 470-510 nm were suitable for the growth and development for A. roxburghii shoots while for A. annamensis, λ = 430- 470 nm were most suitable for budding and λ= 470-510 nm for shoot growth.Sử dụng nguồn chiếu sáng từ đèn LED (Light Emitting Diode) trong nhân giống in vitro hai loài Kim tuyến nói trên nhằm tối ưu hoá điều kiện nuôi cấy, tiết kiệm chi phí sản xuất, đặc biệt là điện năng tiêu thụ cho chiếu sáng và điều hoà. Các nguồn chiếu sáng đèn LED ở bốn dải bước sóng khác nhau λ= 430-470 nm, λ=470-510 nm, λ= 510-5160 nm và λ= 560- 600 nm
đèn huỳnh quang làm đối chứng với cường độ chiếu sáng là 40 μmol/m2/s photon được sử dụng để nghiên cứu những ảnh hưởng của chúng lên sự sinh trưởng và phát triển của hai loài Kim tuyến A. annamensis và A. roxburghii. Kết quả sau 8 tuần nuôi cấy cho thấy đèn LED có λ= 470-510 nm thích hợp cho sự sinh trưởng và phát triển của chồi A. roxburghii, đèn LED có λ= 430-470 nm thích hợp cho sự sinh chồi và đèn LED có λ= 470-510 nm thích hợp cho sự phát triển chồi A. annamensis.

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