Tạo chủng vi tảo Chlamydomonas reinhardtii tái tổ hợp mang gen mã hóa protein VP28 của virus gây bệnh đốm trắng trên tôm

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Tác giả: Mouradov Aidyn, Diễm Hồng Đặng, Duy Kháng Đinh, Văn Quyền Đồng, Thị Thu Hà, Minh Hường Nguyễn, Thị Hoa Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 571.29 Microorganisms, fungi, algae

Thông tin xuất bản: Sinh học, 2018

Mô tả vật lý: 92-99

Bộ sưu tập: Metadata

ID: 492066

Thêm vào giỏ

Virus gây hội chứng đốm trắng (WSSV) là tác nhân hàng đầu gây chết tôm ở các trang trại nuôi tôm trên thế giới. Ở Việt Nam, WSSV đã được xác định là một trong các tác nhân gây tổn thất lớn nhất cho ngành nuôi tôm. Gen mã hóa protein VP28 được cải biến mã di truyền cho phù hợp với hệ thống biểu hiện C. reinhardii và đưa vào hệ gen C. reinhardtii bằng phương pháp xung điện. Các chủng C. reinhardtiii tái tổ hợp được chọn lọc bằng phương pháp PCR và giải trình tự gen
sự biểu hiện của VP28 ở mức độ phiên mã được đánh giá mã bằng phương pháp RT-PCR. Kết quả đã tạo được các chủng C.reinhardtii tái tổ hợp biểu hiện VP28. Các nghiên cứu đang được thực hiện nhằm phát triển vaccine theo đường ăn phòng bệnh đốm trắng ở tôm.White spot syndrome virus (WSSV) is the leading cause of shrimp mortality in farms all over the world. In Vietnam, for the last five to ten years, WSSV has always been among the top causes of diseases and loss in our shrimp aquaculture. VP28 and VP26 are two capsid proteins of WSSV that commonly used as biomarkers for diagnosis and target antigens for vaccine against WSSV. Recombinant VP28 (rVP28) has been studied and expressed in various expression systems including E. coli, yeast and baculovirus. rVP28 expressed in these systems showed effective protection against WSSV in shrimps, though there remains limitation on expression efficiency and safety. Recently, green microalgae Chlamydomonas reinhardtii has been widely used to express pharmaceutical proteins and edible vaccines for aquaculture thanks to its advantages as a safe and efficient host. C. reinhardtii is also used as nutrious natural food for shrimps due to its benefits towards shrimp health and growth. In this study, the codons of vp28 gene was adapted and chemically synthesized, and transformed into the nucleus genome of C. reinhardtii using electroporation. The presence of a codon optimized vp28 gene in C. reinhardtii genome was confirmed by colony PCR and sequencing
and its expression level was examined by RT-PCR. These results proved our success in creating transgenic C. reinhardtiii expressing rVP28 and set the foundation for our future research on edible vaccine against WSSV for shrimp.

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