Giải trình tự và phân tích toàn bộ hệ gen chủng virus nhược độc Hanvet1.VN sử dụng trong sản xuất vaccine phòng hội chứng rối loạn sinh sản và hô hấp ở lợn

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Tác giả: Duy Kháng Đinh, Văn Quyền Đồng, Thị Thu Hà, Hữu Vũ Nguyễn, Thanh Ba Nguyễn, Thị Hoa Nguyễn, Thị Nga Nguyễn, Long Thành Tô, Thị Thu Hiền Trần, Văn Khánh Trần, Thị Hiền Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 641.664 Food and drink

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 51-57

Bộ sưu tập: Metadata

ID: 492076

Thêm vào giỏ

Giải mã và phân tích toàn bộ hệ gen chủng PRRSV Hanvet1.VN. RNA tổng số được tách chiết từ chủng Hanvet1.VN, sử dụng RT-PCR khuếch đại 15 phân đoạn riêng rẽ của toàn bộ hệ gen. Kết quả nghiên cứu cho thấy chủng vaccine Hanvet1.VN nhược độc có đặc tính kháng nguyên sinh miễn dịch phòng hộ tương đồng cao so với chủng PRRSV lưu hành trong nước, gần gũi so với chủng lưu hành ở Trung Quốc nhưng có sự khác biệt khá xa so với chủng cổ điển type II Bắc Mỹ (VR2332). Vì vậy khi nhập vaccine, đặc biệt từ các nước Âu-Mỹ để dự phòng bệnh PRRS cũng nên xem xét cả nguồn gốc chủng PRRSV sử dụng trong sản xuất vaccine.The porcine reproductive and respiratory syndrome virus (PRRSV) attenuated strain Hanvet1.VN has been developed by the Pharmaceutical and Veterinary Material J.S.C (HANVET) by passaging HY-2010 strain on MARC-145 cells for 80 passages and used for PRRS vaccine production. In this study, we sequenced and analyzed the whole genome of the attenuated Hanvet1.VN strain. The total RNA was extracted from the Hanvet1.VN strain, RT-PCR was used for amplification of 15 separate segments of the whole genome. The amplified segments were cloned into the pCR2.1 vector and sequenced by Sanger sequencing. The sequences were analyzed with BioEdit and DNA Star Software. The results showed that, GP5 of the Hanvet1.VN attenuated strain had 100% identity in amino acid (aa) sequences with one of the pathogenic Vietnamese strain isolated in Quang Nam Province and had 98% identity with that of the Chinese 07NM strain. However, the identity of aa sequence of the Hanvet1.VN GP5 was much lower in the comparison with GP5 of VR2332, and it was only 87%. The MP and NP proteins were highly conserved compared with pathogenic strains circulating in Vietnam (07QN) and China (07NM) (99-100%, respectively). The other eight proteins of the Hanvet1.VN strain showed changes from 1.2% in NP1a to 3.9% in GP2 compared with the 07QN strain. However, the aa identity of all Hanvet1.VN proteins were very low when compared with proteins of PRRSV type II strain (North American strain, VR2332), ranged from 86.25% to 97.7%. Our results showed that the Hanvet1.VN attenuated vaccine strain had protective immunogenicity similar to that strain circulating in Vietnam closely related to a strain from China but different from the type II North American strain VR2332. Hence, for importing PRRSV vaccine, especially from American or Europe Countries, antigenic compatibility of the PRRSV vaccine and strains circulating in Vietnam should be concerned in PRRSV vaccine production .

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