Tìm hiểu đa dạng di truyền cá lăng chấm (Hemibagrus guttatus Lacepede, 1803) bằng chỉ thị phân tử microsatellite

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Tác giả: Hà My Bùi, Hữu Đức Nguyễn, Thị Hương Nguyễn, Thị Thúy Hà Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 571.85 Developmental genetics

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 59-65

Bộ sưu tập: Metadata

ID: 492077

Thêm vào giỏ

Ba chỉ thị microsatellite được sử dụng để tìm hiểu đặc điểm di truyền của 4 quần đàn cá Lăng chấm (3 quần đàn tự nhiên tại Tuyên Quang, Phú Thọ, Hà Giang và 1 quần đàn cá bố mẹ nuôi giữ tại Hải Dương). Các chỉ thị này có mã số truy cập lần lượt là KJ873116, KJ873117 và NC023976. Ba vị trí microsatellite đều thể hiện tính đa hình cao, với tổng số 16 allele trên cả 3 locus, lần lượt là 5, 5 và 6 allele tương ứng với vị trí locus HM7, HM8 và SS1. Quần đàn cá Lăng chấm thu ở Hà Giang có tổng số allele cao hơn so với ba quần đàn thu ở Tuyên Quang, Phú Thọ và Hải Dương. Số allele quan sát lớn hơn số allele hiệu quả trên tất cả các vị trí phân tích và tại mỗi locus đều xuất hiện những allele với tần số rất thấp. Mức dị hợp tử quan sát cho giá trị lớn hơn mức dị hợp tử kì vọng.Bagrid catfish (Hemibagrus guttatus Lacepede, 1803) is a wild species of high economic value in Northern Vietnam. Artificial reproduction of bagrid catfish requires sources of quality fingerlings in terms of genetics. In fact, bagrid catfish is endangered due to overhunting. Until now, studying on bagrid catfish was mainly focused on biology characterictics and artificial breeding. In this study, three microsatellite markers were used to assess the genetic characteristics of four bagrid catfish populations (three wild populations collected in Tuyên Quang, Phu Tho, Ha Giang and a cultured population in Hai Duong). These markers were registed Genbwith the code of KJ873116, KJ873117 and NC 023976 for HM7, HM8 and SS1, respectively. All of the loci showed high level of polymorphism with 16 alleles in total which were 5 at locus HM7, 5 at locus HM8, and 6 at locus SS1. Total allele number in population collected in Ha Giang was higher than that of Tuyen Quang, Phu Tho and Hai Duong populations. The mean of number of observed alleles (Na = 3,83 ± 0,24) was higher than the mean number of effective alleles (Ne = 2,14 ± 0,13) and low frequency alleles (<
0,1) were observed in all loci. The value of the expected heterozygosity (He = 0,38- 0,63) was lower than that of the observed heterozygosity (Ho = 0,51-0,71). FIS value was low and the genetic differences between four populations was insignificant as FST <
0,05. The results provide useful information for breeding program and conservation of the bagrid catfish in the future

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