Ứng dụng kỹ thuật xác định kiểu gen bằng giải trình tự (GBS) để sàng lọc các đa hình nucleotide đơn (SNPs) liên quan đến tính trạng tăng trưởng ở tôm sú (Penaeus monodon)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Huyền Thương Ma, Đăng Tôn Nguyễn, Quyết Tâm Nguyễn, Thị Minh Thanh Nguyễn, Văn Hảo Nguyễn, Văn Sáng Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 016.57 Bibliographies and catalogs of works on specific subjects or in specific disciplines

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 75-85

Bộ sưu tập: Metadata

ID: 492078

Thêm vào giỏ

Nowadays, the black tiger shrimp (Penaeus monodon) farming in Vietnam still have to face a lot of problems, especially in the Country's Shrimp Broodstock Production. To overcome these problems, we need a strategy for development of domesticated stocks of P. monodon and rational breeding programs for improved growth and disease resistance. Marker-assisted selection is a procedure that has been developed to avoid these problems associated with phenotypic selection, replacing the selection of the phenotype by selection using markers linked with quantitative trait loci (QTL). Application of next generation sequencing (NGS) associated with QTL to discover important molecular markers has been applied in many laboratories. In this study, we used genome typing by sequencing (GBS) with NGS for screening the SNPs related with growth trait to create a database for father study on the black tiger shrimp fast growth selection. Based on the putative genome sequences created by de novo assembly, 2887 SNPs have been screened, among them, 1799 SNPs appeared only in the fast growth population. Among the 287 annotated contigs, the two contigs were homologous with myosin heavy chain (MHC) associated with growth trait in crustacean species, contig83953 andcontig260347 were homologous with MHCa and MHC1, respectively. The two SNPs Contig83953:g.20T>
C and Contig260347:g.19G>
A were indicated. We hope that, these results will contribute to the black tiger shrimp genome database for further study to exploit the molecular markers for black tiger shrimp breeding and selections.Sử dụng kỹ thuật xác định kiểu gen bằng giải trình tự GBS với công nghệ NGS của Illumina để sàng lọc các đa hình nucleotide đơn liên quan đến tính trạng tăng trưởng nhằm tạo cơ sở dữ liệu cho các nghiên cứu chọn giống tôm sú hướng đến tính trạng tăng trưởng nhanh. Dựa trên hệ gen tham chiếu tạm thời của tôm sú được thiết lập de novo, tổng số 2.887 SNPs đã được sàng lọc, trong đó có 1.799 SNPs chỉ xuất hiện ở nhóm tôm sú lớn nhanh.Trong số 287 contig được chú giải ở nhóm tôm sú lớn nhanh có hai contig chứa SNPs có sự tương đồng với gen mã hóa protein myosin heavy chain (MHC) liên quan đến tính trạng tăng trưởng ở họ giáp xác, trong đó contig83953 tương đồng với MHCa và contig260347 tương đồng với MHC1.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR