Khảo sát nano bạc làm chất khử trùng mẫu mới trong nhân giống vô tính cây African violet (Saintpaulia ionantha H. Wendl.)

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Tác giả: Mạnh Cường Đỗ, Bảo Trinh Dương, Tấn Nhựt Dương, Thanh Tùng Hoàng, Thị Thu Hiền Lê, Hoài Châu Nguyễn, Phúc Huy Nguyễn, Quốc Luận Vũ, Thị Hiền Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 614.48 Disinfection, fumigation, sterilization

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 87-97

Bộ sưu tập: Metadata

ID: 492079

Thêm vào giỏ

Các chất khử trùng thông dụng và nano bạc đã được sử dụng để khử trùng mẫu cấy African violet (Saintpaulia ionantha H. Wendl.) với dãy nồng độ, thời gian khử trùng khác nhau để khảo sát khả năng khử trùng và cảm ứng mẫu cấy của nano bạc trong giai đoạn khử trùng mẫu. Sau khi khử trùng, tiến hành theo dõi và đánh giá sự sinh trưởng, phát triển của mẫu cấy qua các giai đoạn khác nhau. Kết quả cho thấy, mẫu cấy được khử trùng bằng nano bạc ở nồng độ 0,05% trong 15 phút cho hiệu quả tốt nhất mà không có tác động xấu đến sự sinh trưởng và phát triển của mẫu cấy. Nano bạc kích thích sự cảm ứng của mẫu cấy.Surface sterilization is one of the most important steps in preparation of explants for micropropagation, because microbial contaminations present a major challenge to the initiation and maintenance of viable in vitro cultures. Most of popular surface disinfectants are considered as highly toxic influence either directly or indirectly to health and environment. Previous studies have demonstrated that toxicity of nano silver (Ag nanoparticles) can destroy effectively microorganisms but it is safe for human health. So, the silver nanoparticles have been widely used in different fields of life, such as medicine, pharmaceuticals, cosmetics, biology and agriculture. However, reports on the effect of silver nanoparticles for surface sterilization of plant explants are still limited. Nano silver and typical disinfectants were tested for sterilization of African violet (Saintpaulia ionantha H. WENDL.), by varying their concentration and time of exposure. The aim of this study was to examine sterilization capacity and explant morphogenesis when using nano silver in micropropagation. After decontamination step, we evaluated the growth and development of explants in different stages of the micropropagation process of African violet. The results indicated that the treatment using nano silver agent at concentration of 0.05% for 15 minutes was the best for controlling the infection. Nano silver could be used to replace the commonly used decontamination substances without causing adverse effects on plant growth and development. This is the first report on in vitro establishment using nano silver to reduce bacterial infections and the growth and development of African violet (Saintpaulia ionantha H. WENDL.).

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