Phân tích tổng quát dựa trên hệ gen học của họ gen RBOH ở cây đậu tương (Glycinee Max L. Merr.)

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Tác giả: Phương Thảo Nguyễn, Đăng Quang Ông

Ngôn ngữ: vie

Ký hiệu phân loại: 635.655 *Soybeans

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 107-117

Bộ sưu tập: Metadata

ID: 492080

Thêm vào giỏ

Áp dụng phương pháp phân tích tổng quan dựa trên hệ gen học cho họ gen Rboh trên hệ gen của cây đậu tương [(Glycine max L. Merr.)]. Sử dụng trình tự hệ gen chất lượng cao, chúng tôi đã xác định được 17 gen Rboh ở cây đậu tương. Các gen Rboh này phân bố khá đồng đều trải dài trên 14 trong tổng số 20 nhiễm sắc thể của cây đậu tương. Số lượng gen GmRboh gần như gấp đôi ở các loài thực vật là kết quả từ sự tiến hóa trong hệ gen của cây đậu tương. Phân tích cây phát sinh loài cho thấy các gen Rboh của đậu tương được chia thành sáu nhóm, và ở mỗi nhóm, cấu trúc gen và sự phân bố các vùng chức năng có tính bảo tồn cao. Ngoài ra, phân tích biểu hiện gen dựa trên dữ liệu RNA-seq có thể nhận thấy 17 gen Rboh của đậu tương biểu hiện khác biệt nhau trên 14 mẫu mô và biểu hiện nhiều nhất ở rễ và nốt sần.Plant respiratory burst oxidase homologs (RBOHs), also known as NADPH oxidase (NOX) have involved as a key producers of reactive oxygen species (ROS) in a wide range of biological processes, including plant growth, development, and abiotic stress responses. In this work, a total 17 soybean RBOH encoding genes (GmRBOHs) were identified. Furthermore, their domain architecture, chromosomal distribution, gene structure, gene duplication, and phylogenetic analysis were carried out. Phylogenetic tree divided the RBOHs from soybean, another legume plants (Phaseolus vulgaris), and Arabidopsis into six subfamilies, with soybean RBOH genes distributed in subfamily I to VI. All RBOH proteins of soybean contained four major domains, which focused on EF-hands domains with Ca2+-binding residues provided more insights into function of RBOH proteins in soybean. The differential expression pattern using RNA-seq of 17 RBOH genes in soybean indicated that most of the genes express in root and nodule
among them, GmRboh9 and GmRboh12 have the highest expression. The analysis presented provides a scientific basis for further research on function of RBOH genes in soybean

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