Vi nhân giống lan nhất điểm hoàng (Dendrobium heterocarpum Lindl.)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Thắm Đặng, Văn Khiêm Đinh, Niê Bing H'Yon, Thị Thanh Hằng Nguyễn, Văn Duy Nông, Văn Hợi Quách, Thái Vinh Trần, Kim Công Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 581.3 +Genetics and evolution

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 127-135

Bộ sưu tập: Metadata

ID: 492081

Thêm vào giỏ

Dendrobium heterocarpum Lindl. is an endangered species which is currently used as ornamental pot plant for its beautiful flowers. An increase in collection for trade or any other purposes may lead to a dramatic decrease in the population of this species, thus becoming rare or endangered species in the near future. In this study, effects of plant growth regulators (BA, NAA, IAA, IBA, TDZ) and natural supplements (carrot, potato, and banana extracts) on protocorm like bodies (PLBs) formation
growth and development of shoot
and root regeneration of D. heterocarpum Lindl. as well as type of substrates on acclimatization and growth of seedlings were investigated. The results showed that PLBs formation was optimal on MS medium supplemented with 2.0 mg/L BA and 1.0 mg/L NAA (7.11 PLBs/explant
PLBs formation percentage of 68.9%) or MS medium supplemented with 1 mg/L TDZ and 0.5 mg/L NAA (7.29 PLBs/explant
PLBs formation percentage of 75.53%). For subculture, MS medium supplemented 1.5 mg/L BA and 60 g/L banana extract (22.40 shoots/explant
shoot length of 2 cm) was the most suitable for shoot regeneration and growth. Additionally, root formation was the most suitable on ½ MS medium supplemented with 1 mg/L NAA (4.4 roots/shoot
root length of 3.12 cm
root formation of 95.56%). Finally, the sufficiently rooted plantlets were transferred to greenhouse for hardening. After 60 days, coconut fiber substrate was the most suitable for seedling growth and development (with survival rate of 97.78%, root number of 5 and shoot length of 3.4 cm). The results of propagation in vitro Dendrobium heterocarpum Lindl. contribute to conservation and sustainable development as well as towards the rapid multiplication of seedlings for commercial commercialization of this wild orchid speciesKết quả vi nhân giống lan nhất điểm hoàng (Dendrobium heterocarpum Lindl.) cho thấy, môi trường thích hợp cho sự hình thành PLB là MS bổ sung 2 mg/L BA và 1,0 mg/L NAA (7,11 PLB/mẫu
68,9% mẫu tạo PLB) hoặc môi trường MS bổ sung 1 mg/L TDZ với 0,5 mg/L NAA (7,29 PLB/mẫu
75,53% mẫu tạo PLB). Môi trường nuôi cấy MS bổ sung 1,5 mg/L BA và môi trường nuôi cấy MS bổ sung 60 g chuối chín/lít đều phù hợp cho sự sinh trưởng và phát triển của chồi cây. Môi trường thích hợp cho sự tạo rễ in vitro là: ½ MS bổ sung 0,5 mg/L NAA. Sau 60 ngày chuyển cây con in vitro ra ngoài vườn ươm, kết quả thí nghiệm cho thấy giá thể thích hợp nhất là giá thể dớn (5,0 rễ/mẫu
chiều dài rễ 3,4 cm
tỉ lệ sống 97,78%).

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR