Đánh giá an toàn sinh học của phức hợp MgAl LDH - Anacardic acid trên cây rau cải ngọt (Brassica integrifolia) và chuột

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Vân Anh Lê, Hữu Hùng Nguyễn, Thị Như Quỳnh Nguyễn, Tiến Thắng Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 571.952 Physiology and related subjects

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 319-325

Bộ sưu tập: Metadata

ID: 492088

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Đánh giá ảnh hưởng của vật liệu nano LDH, anacardic acid và phức hơp L-A trên sự tăng trưởng và phát triển của cây rau cải ngọt, sự tồn dư hoạt chất anacardic acid khi cây rau cải ngọt được xử lý bằng phức hợp L-A trong 6 ngày theo dõi bằng phương pháp HPLC và độc tính cấp trên chuột thông qua giá trị LD50. Kết quả cho thấy, vật liệu nano LDH và phức hợp L-A không ảnh hưởng đến các chỉ số sinh trưởng của cây cải ngọt so với đối chứng ở liều dùng kiến nghị để diệt ấu trùng gây hại 0,148 mg/mL. Kết quả xác định độc tính cấp LD50 trên chuột của anacardic acid là 2378,41 mg/kg thể trọng, trong khi với liều anacardic là 2553 mg/kg thể trọng được gắn lên vật liệu nano LDH không có con chuột nào chết sau 9 ngày theo dõi. Vậy, khi anacardic acid được gắn lên vật liệu nano LDH thì độc tính của nó giảm. Đồng thời kết quả thử nghiệm độc tính của vật liệu nano LDH trên chuột với lượng 6000 mg/kg không có con nào chết hoặc có biểu hiện bất thường trong thời gian khảo sát. Điều này chứng tỏ LDH và L-A không gây độc tính cấp trên chuột thí nghiệm.MgAl layered double hydroxides adsorbed anacardic acid (L-A complex) nanomateial used as a biological pesticide, could kill cutworms larvae (Spodoptera litura). This study assessed the effect of the MgAl layered double hydroxides, anacardic acid, and L-A complex on growth and development of sweet mustard trees, their toxicity on mice and the anacardic acid substance residues of L-A complex on sweet mustard trees (Brassica integrifolia) using HPLC method. The results showed that the MgAl layered double hydroxides and L-A complex did not affect the growth indexes including body height, root length, fresh weight, dry weight and chlorophyll concentrations of sweet mustard when killing larvae dose of 0,148 mg/mL was applied to the plants. The toxicity evaluation on mice indicated LD50 values of anacardic acid was 2378,41 mg/kg body weight, while no mice died from using L-A complex with anacardic acid concentration of 2553 mg/kg body weight. These results indicated that the toxicity of anacardic acid was reduced as using anacardic acid with MgAl layered double hydroxides in comparison with using anacardic acid alone. In testing the toxicity of free LDH, at dose applying on mice 6000 mg/kg body weight, no death mice or any abnormal behaviors of them was recorded. The result also showed that the anacardic acid residues in mustard leaves decreased from 40,6 mg/kg to 15,7 mg/kg leaves after 6 days. Therefore, all the results indicated that LDH, anacardic acid and L-A complex had no acute toxicity in mice and negative effect on growth and development of mustard plant. Prospectively, anacardic acid and L-A complex can be used as biological pesticides and safety materials in agriculture

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