Nghiên cứu tách chiết và tác dụng tăng cường miễn dịch của các polysaccharide từ lá cây thuốc xuân hoa Pseuderanthemum palatiferum (NEES) Radlk

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Tác giả: Văn Trường Lê, Thị Mai Phương Nguyễn, Thị Thanh Hương Nguyễn, Thị Vân Anh Trần, Hoài Bắc Võ

Ngôn ngữ: vie

Ký hiệu phân loại: 571.962 Physiology and related subjects

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 327-335

Bộ sưu tập: Metadata

ID: 492089

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Pseuderanthemum palatiferum (Ness) Radlk is a tree native of Vietnam with various use in traditional medicine. It has been used for the treatment of many diseases including wound, colitis, stomach-aches, trauma, high blood pressure, nephritis, diarrhoea. However, the research on effective constituents from P. palatiferum has mainly been focused on small-molecular compounds. Some compounds: flavonoids, phytol, palmitic acid, ß-sitosterol, triterpenoid saponin, stigmasterol and salicylic acid have been detected in leaves. Polysaccharides have attracted great attentions for its benefits to human health. Polysaccharides from natural sources have diverse anti-inflammatory, anticoagulant and wound healing activities. In this study, we extracted and determined the polysaccharide content from the leaves of P. palatiferum plant. The polysaccharide content in P. palatiferum leaves was 8.2% (± 0.65) in dry weight. The appropriate polysaccharide extraction conditions were determined as: 25% ethanol, material/solvent ratio (1g/10ml), extracted temperature of 70°C, extraction time 12 hours. The polysaccharide composition was purified by TCA 10%, with a purity of 77.8% (± 1.19). The immunostimulatory activities of polysaccharide XH were also examined in cyclophosphamide (CY)- induced immunosuppressed mice. Mice treated with polysaccharide XH exhibited increased white blood cell counts (p <
0.05) compared with immunosuppressed mice. These results indicate that polysaccharide XH from P. palatiferum can enhance immune function in CY-induced immunosuppressed mice.Tách chiết, tinh sạch sơ bộ và xác định được tác dụng tăng cường miễn dịch của polysaccharide chiết xuất từ cây thuốc xuân hoa (Pseuderanthemum). Kết quả cho thấy hàm lượng polysaccharide trong lá cây xuân hoa đạt 8,2% (± 0,65) trọng lượng khô. Các điều kiện chiết rút polysaccharide thích hợp là dung môi ethanol 25%, tỷ lệ nguyên liệu/dung môi (1g mẫu khô/10ml), nhiệt độ chiết rút 70°C, thời gian chiết rút 12 giờ. Chế phẩm polysaccharide đã được tinh sạch bằng TCA 10%, có độ sạch đạt 77,8% (± 1,19 %) và hiệu suất thu nhận chế phẩm polysaccharide đạt 1,79 % (± 0,24).

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