Nghiên cứu chuyển gen OsNAC45 liên quan tới tính chịu hạn vào cây ngô Zea Mays

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Tác giả: Thị Thu Hương Bùi, Lệ Quyên Cao, Thị Thu Huệ Huỳnh, Duy Phương Nguyễn, Thu Hằng Phạm, Xuân Hội Phạm

Ngôn ngữ: vie

Ký hiệu phân loại: 571.962 Physiology and related subjects

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 465-472

Bộ sưu tập: Metadata

ID: 492099

Thêm vào giỏ

Plants response to abiotic stresses by initiating a series of processes including the activation of transcription factors that can regulate expression of various stress-responsive and adaptive genes. NAC family (NAM, ATAF1/2, CUC2), which is the largest plant transcription factor family, plays an important role in the development and stress responses of plants. OsNAC10 gene fragment has 1080 nucleotide containing an open reading frame (OFR) encoding for protein OsNAC45. OsNAC45 is expressed predominantly in roots and leaves and induced by drought, high saliniy, low temperature and abscisic acid. Overexpression of OsNAC45 in rice increased the plant tolerance to drought and high salinity. In this study, recombinant expression vector containing OsNAC45-encoding sequence under the control of the Arabidopsis stress-inducible Rd29A promoter was designed and successfully transformed into model maize plants via Agrobacterium tumefaciens bacteria. The genotype of regeneration plants was identified by PCR and Real-time pPCR. Using semiquantitative RT-PCR, the expression of transgene OsNAC45 in some T0 transgenic plants were detected. These results provide a basis for the study of the function of OsNAC45 in drought responses and for the development of drought stress tolerant crops.Thiết kế cấu trúc biểu hiện gen OsNAC45 liên quan tới đáp ứng stress hạn của lúa Mộc tuyền, điều khiển bởi promoter hoạt động cảm ứng stress Rd29A. Cấu trúc biểu hiện gen đã được chuyển nạp vào cây ngô mô hình thông qua vi khuẩn A. tumefaciens với hiệu suất chuyển gen đạt 0,17%. Các dòng ngô chuyển gen đã được sàng lọc bằng phương pháp PCR xác định sự có mặt và Realtime PCR xác định số bản sao của cấu trúc chuyển gen. Bằng kĩ thuật RT-PCR bán định lượng mRNA, sự biểu hiện của OsNAC45 đã chứng minh được trong các dòng ngô chuyển gen T0 được xử lý hạn nhân tạo. Các kết quả nghiên cứu này là tiền đề cho các nghiên cứu sâu hơn về chức năng của OsNAC45, từ đó hướng tới việc cải tạo tính trạng chịu hạn của các giống cây trồng nhờ công nghệ chuyển gen thực vật.

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