Vai trò của nano bạc trong nâng cao tần suất hình thành tế bào đơn cây hoa salem (Limonium sinuatum (L.) Mill)

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Tác giả: Mạnh Cường Đỗ, Tấn Nhựt Dương, Thanh Tùng Hoàng, Thành Long Lê, Thị Nhật Linh Nguyễn, Thị Bích Phượng Trương, Quốc Luận Vũ, Thị Hiền Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 620.5 Nanotechnology

Thông tin xuất bản: Công nghệ Sinh học, 2018

Mô tả vật lý: 481-490

Bộ sưu tập: Metadata

ID: 492100

Thêm vào giỏ

In this research, nanosilver with different concentrations and treatment time was used to sterilize infection agents and induce initial explants which were used as materials for cell suspension culture of Limonium sinuatum. The result showed that leaf explants sterilized with 0.2 g/L nanosilver for 20 minutes had highest effect (live rate 73.33%) comparing to HgCl2 and Ca(ClO)2 (56.66% and 64.44%, respectively). In addition, the leaf explants which were treated with nanosilver and cultured in ½ MS medium supplemented with 20 g/L sucrose, 1 mg/L picloram and 2.5 g/L gelrite also induced calli (friable calli, milk white color, embryogenic callus structure). Moreover, cell suspension formation process from these calli was also observed highest on the 20th day (49,088 cells/mL) in liquid shaking culture condition comparing to control treatment (19,361 cells/mL) when they were cultured on similar medium combined with 1.2 mg/Lnanosilver. These cells had the best growth, development and regeneration from the 16th day to the 20th day. The ability of shoot and callus regeneration was highest (67.77%) in ½ MS medium which was supplemented with 20 g/L sucrose, 1 mg/L zeatin, 2.5 g/L gelrite and 1.6 mg/L nanosilver compared with control treatment having no nanosilver (40.00%). This research showed that in micropropagation of Limonium sinuatum, nanosilver was proved to be an effective agent for sterilization, explant induction, cell suspension origination, and callus and shoot regeneration. Beside that, nanosilver had negative impact on the development of explants when it was used with high concentration for extended period.Nghiên cứu vai trò của nano bạc trong nâng cao tần suất hình thành tế bào đơn cây hoa salem (Limonium sinuatum). Kết quả cho thấy việc sử dụng nano bạc nồng độ 0,2 g/L trong 20 phút có thể thay thế các chất khử trùng thông dụng (HgCl2, Ca(ClO)2) trong vi nhân giống cây hoa salem. Bên cạnh đó, nano bạc còn có tác dụng kích thích mẫu cấy cảm ứng nhanh, tác động đến sự phát sinh hình thái và hoàn toàn không gây ra bất kỳ tác động tiêu cực nào đến mẫu cấy. Ngoài ra, nano bạc nồng độ 1,2 mg/L thích hợp cho sự phát triển huyền phù tế bào. Trong khi khả năng hình thành, sinh trưởng và phát triển của chồi là 1,6 mg/Lnano bạc.

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