Acute kidney injury (AKI) represents a concerning health challenge that alters renal structure and function. Ferroptosis has been identified as a cell death mechanism involved in AKI. Cisplatin is a widely used antineoplastic agent, but AKI is a serious limitation to its clinical use. We explored the effect of roflumilast on cisplatin-induced AKI. Wistar rats were divided into 3 groups (n = 7 per group)
control group, cisplatin group rats received a single injection (7 mg/kg, i.p.), and roflumilast/cisplatin group: roflumilast was given two days earlier and one hour before cisplatin injection and was continued for the following five days. Kidney histological structure and renal function were assessed. Additionally, the effect on the antioxidant reduced glutathione (GSH) and malonaldehyde (MDA), the lipid peroxidation product, were investigated. Ferroptosis regulators such as xCT subunit of system Xc