Kết quả chọn tạo giống lúa chịu mặn SHPT15 bằng phương pháp chọn dòng cá thể sử dụng chỉ thị phân tử

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Đức Hà Chu, Thị Mai Lương Khuất, Hà Minh Lê, Hùng Lĩnh Lê, Huy Hàm Lê, Thúy Kiều Tiên Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 631.52 Production of propagational organisms and new varieties

Thông tin xuất bản: Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý: 45612

Bộ sưu tập: Metadata

ID: 492287

Thêm vào giỏ

Tóm tắt
Chủ đề

Chọn dòng cá thể sử dụng chỉ thị phân tử (MAS) là một trong những công cụ đắc lực để tạo ra giống lúa mới có khả năng ứng phó với biến đổi khí hậu. Trong nghiên cứu này, dòng lúa SHPT15 đã được chọn tạo bằng phương pháp MAS từ phép lai Bắc Thơm số 7 (BT7) × FL478 (giống cho gen mang locus gen Saltol). Cụ thể, SHPT15, chọn dòng cá thể từ thế hệ BC3F6, đã được kiểm tra có mặt của locus gen Saltol ở trạng thái đồng hợp tử bằng hai chỉ thị phân tử. Đánh giá kiểu hình cho thấy dòng lúa SHPT15 có khả năng chịu được mặn 6‰ trong điều kiện nhân tạo ở giai đoạn cây non trong 15 ngày xử lý. Đánh giá trong hai vụ tại xã Giao Châu, huyện Giao Thủy, tỉnh Nam Định đã cho thấy, SHPT15 có khả năng thích ứng với canh tác tại các tỉnh phía Bắc. Dòng lúa SHPT15 có các đặc tính nông sinh học tốt, thời gian sinh trưởng ngắn, tương đương so với BT7. Trong điều kiện vụ Xuân và vụ Mùa, năng suất thực thu của SHPT15 cao hơn so với BT7, đạt 6,37 tấn/ha (vụ Xuân) và 6,14 tấn/ha (vụ Mùa). Ngoài ra, dòng lúa SHPT15 cũng thể hiện khả năng chống chịu một số sâu bệnh hại chính ở mức khá, tương đương so với BT7. Kết quả của nghiên cứu này sẽ tạo tiền đề quan trọng cho việc khảo nghiệm sinh thái dòng lúa SHPT15 tại các tỉnh phía Bắc.Marker-assisted selection (MAS) has been considered as one of the most effective tools for the construction of rice varieties adapted to climate change. In this study, we successfully created the new rice line, namely SHPT15 from Bac Thom No 7 (BT7) × FL478 (donor harboring Saltol) by the MAS approach. Particularly, the SHPT15 line was obtained from the BC3F6 generation and validated the occurrence of the homozygous Saltol locus gene by molecular markers. Our results also demonstrated that SHPT15 could resist high salinity conditions (6‰) at the seedling stage for 15 days. Next, our author testings in two seasons in Giao Chau Commune, Giao Thuy District, Nam Dinh Province found that SHPT15 has potentially developed in the Northern provinces. More specifically, SHPT15 had some good agronomical traits, such as the short growth duration, as similar to the BT7 varitety. Interestingly, the yields of SHPT15 have been found to be significantly higher than BT7 (6.37 tons/ha in the Spring season and 6.14 tons/ha in the Summer season). Additionally, SHPT15 also exhibited a high tolerance to major insects/diseases in the field. Taken together, our results could provide a solid foundation for further ecological testings of SHPT15 in the Northern provinces in Vietnam.

Tạo bộ sưu tập với mã QR