Ảnh hưởng của chất điều hòa sinh trưởng lên sự tăng sinh và phát sinh hình thái của mô sẹo có nguồn gốc từ tử diệp cây bá bệnh (Eurycoma longifolia)

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Tác giả: Đăng Giáp Đỗ, Thị Dược Nguyễn, Trọng Tuấn Trần, Thị Hương Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại: 571.849 +Cell growth

Thông tin xuất bản: Tạp chí khoa học công nghệ và thực phẩm, 2018

Mô tả vật lý: 65-75

Bộ sưu tập: Metadata

ID: 492366

Thêm vào giỏ

Bá bệnh hay còn gọi là mật nhân (Eurycoma logifolia) là loại dược liệu quý thuộc họ thanh thất (Simaroubaceae). Công dụng chính yếu của cây này là điều trị sốt rét và tăng cường chức năng sinh lý nam. Cây bá bệnh có chứa nhiều hợp chất có hoạt tính sinh học như quassinoid, alkaloid, các dẫn chất squalene và steroid. Hiện nay, nhiều nghiên cứu về hợp chất thứ cấp và công dụng của cây này đã được thực hiện. Tuy nhiên, các nghiên cứu về nuôi cấy in vitro vẫn còn hạn chế. Trong nghiên cứu này, ảnh hưởng của chất điều hòa sinh trưởng lên sự tăng sinh khối mô sẹo và sự phát sinh hình thái của mẫu mô sẹo có nguồn gốc từ tử diệp cây bá bệnh được khảo sát. Kết quả thí nghiệm khảo sát sự tăng sinh mô sẹo cho thấy, các mẫu mô sẹo bá bệnh tăng sinh khối cao nhất trong môi trường MS có bổ sung 3,0 mg/L 2,4-D kết hợp với 2,0 mg/L thidiazuron (TDZ) (3,86 g/mẫu). Ở nghiệm thức sử dụng 2,4-D kết hợp với IBA, các mẫu cấy mô sẹo được nuôi cấy trên môi trường có bổ sung 2,4-D kết hợp với IBA đều có sự phát sinh rễ và cảm ứng hình thành phôi vô tính. Kết quả ở nghiệm thức này cho thấy, sự hình thành rễ ở các mẫu mô sẹo cao nhất ở nghiệm thức có bổ sung 0,5 mg/L 2,4-D kết hợp với 4,0 mg/L IBA (5,3 rễ/mẫu). Với chỉ tiêu về số lượng phôi, sự phát sinh phôi vô tính cao nhất ở nghiệm thức chỉ bổ sung 3,0 mg/L IBA (14,3 phôi/mẫu).'Ba benh' or 'Mat nhan', which is a common name of Eurycoma longifolia in Vietnam, is a medicinal herb of the Simaroubaceae family. The main uses of this species are malaria treatment and physiological enhancement. There are many biologically active compounds in this plant, such as quassinoid, β-carboline alkaloid, canthin-6-1 alkaloid, tirucallane triterpene, squalene derivatives, eurycolactone, eurycomalactone, laurycolactone, biphenyl neolignan and steroids. Currently, many studies on secondary compounds and their uses have been done, however, studies on morphogenesis and soma embryogenesis are still limited. In this study, the effects of plant growth regulators on proliferation and morphogenesis of callus derived from cotyledon of Eurycoma longifolia were investigated. The results showed that calli proliferation cultured in 3.0 mg/L 2,4-D combined with 2.0 mg/L TDZ MS medium reach the highest fresh weight (3.86 g/explant). Almost callus explants were cultured in MS medium supplemented with 2,4-D along with IBA also induce roots and somatic embryos. The results of this experiment showed that 0.5 mg/L 2,4-D combined with 4.0 mg/L IBA was suitable for root induction with 5.3 roots per explant. The medium supplemented with 3.0 mg/L IBA was the most suitable medium for somatic embryogenesis with 14.3 embryos per explant.

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