Khảo sát sự sinh trưởng, khả năng kháng oxy hóa và hàm lượng phenolic của cây xạ đen (Ehretia asperula Zoll. & Mor.) in vitro dưới tác động của đèn led

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Quỳnh Loan Lê, Hoàng Dũng Nguyễn, Thị Mỹ Trâm Trần, Trọng Tuấn Trần, Thị Hương Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại: 581.3 +Genetics and evolution

Thông tin xuất bản: Tạp chí khoa học công nghệ và thực phẩm, 2018

Mô tả vật lý: 38-48

Bộ sưu tập: Metadata

ID: 492369

Thêm vào giỏ

Cây xạ đen (Ehretia asperula Zoll. & Mor.) có chứa các hợp chất như flavonoid, saponin triterpenoid, quinon. Nghiên cứu này tìm hiểu vai trò của kinetin đối với khả năng tạo chồi từ mẫu cấy đốt thân ex vitro và ảnh hưởng của đèn LED (light emtting diodes) lên sự sinh trưởng và khả năng kháng oxy hóa, hàm lượng phenolic của mẫu cao chiết cây xạ đen nuôi cấy in vitro. Các mẫu chồi xạ đen ex vitro được nuôi cấy trên môi trường MS có bổ sung KIN ở nồng độ 3,0 mg/L đạt tỷ lệ mẫu cấy hình thành chồi là 100%, số chồi hình thành đạt 1,57 chồi/mẫu. Các mẫu chồi cây xạ đen in vitro đạt chiều cao khoảng 2,0 cm được nuôi cấy trên môi trường MS không bổ sung chất điều hòa sinh trưởng thực vật, được đặt dưới các điều kiện ánh sáng khác nhau, bao gồm: 100% LED đỏ, 100% LED xanh, ánh sáng LED đỏ và LED xanh kết hợp với tỷ lệ 50:50, 70:30, đèn growth light và đèn huỳnh quang được sử dụng làm mẫu đối chứng. Sau 4 tuần nuôi cấy, cây xạ đen in vitro nuôi cấy dưới ánh sáng của đèn growth light có khối lượng tươi, chiều cao cây và số lá thu được cao nhất (tương ứng 1,57 g, 4,46 cm và 9,11 lá). Đồng thời, các mẫu chồi nuôi cấy dưới ánh sáng đèn growth light có hàm lượng phenolic (81,06 mg GAE/g cao chiết) và khả năng kháng oxy hóa (IC50 là 0,1166 mg/L) cao hơn so với các thí nghiệm còn lại. Ngoài ra, mẫu cao chiết xạ đen có sự hiện diện của các nhóm hợp chất thứ cấp khác như carbohydrate, protein-amino acid, chất béo.Ehretia asperula Zoll. & Mor. contains compounds such as flavonoid, saponin triterpenoid, quinon. In this paper, the effects of kinetin on the shoot formation derived from ex vitro shoot and effect LED (Light emitting diode) on the growth plant, antioxidant activity, and total phenolic content were studied. Ex vitro-derived nodes were cultured on MS medium supplemented with KIN at a concentration of 3.0 mg/L, the shoot formation ratio (100%) and shoots per explant (1.57 shoots/explant) were the highest one. The Ehretia asperula shoots of 2,0 cm high were cultured in MS medium without plant growth regulators, and incubated under six different lighting conditions: monochromatic red LED (R), monochromatic blue LED (B), red and blue LED mixtures (50R:50B, 70R:30B), growth light and fluorescent lamp light (control experiment). After 4 weeks of culture, in vitro plants cultured under the growth light condition reached the highest level of fresh weight, plant height and leaf number (1.57 g, 4.46 cm and 9.11 leaves, respectively). In addition, the explants cultured under the growth light conditions showed antioxidant capacity (IC50 = 0.1166 mg/mL) and total phenolic content (81,06 mg GAE/g extract) higher than those cultured under other light conditions. Besides, there are many secondary compounds such as carbohydrate, protein - amino acid, lipid in extract of E. asperula plant.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR