GENERATION STABLE CELL LINE USED FOR PROTEIN EXPRESSION SYSTEM

• Tác giả: Thi Thu Huong Nguyen
• Ngôn ngữ: vie
• Ký hiệu phân loại: 571.84 +Reproduction and growth of cells
• Thông tin xuất bản: Hong Duc University Journal of Science, 2017
• Bộ sưu tập: Metadata
• ID: 492476

Tóm tắt

The ecdysone inducible system was generated for checking the the toxicity of the target gene which was transfected into the cells. The retinoid X receptor (RXR) was transfected into HEK293FT to generate an inducible stable cell line pVgRXR-HEK293FT. For checking the function, the cells transfected with pIND-GFP and induced by 1µM ponasteron A for GFP expression. The fluorescence signals were detected in the transfected and induced cells informed the success of experiment.