The 1,2,4-triazole fungicides are extensively used in agriculture, and their impacts on aquatic organisms by continuous release are increasingly concerned. Aromatase, a rate-limiting enzyme for androgens converting to estrogens, is considered as a potential target for triazole fungicides. To reveal and predict the aromatase inhibition capacity of the existing and future developed triazole fungicides, 23 commonly used 1,2,4-triazole fungicides were used for the evaluation of their inhibitory effects (expressed as the 50% inhibitory concentration (IC