Khảo sát khả năng ức chế nấm Alternaria sp. gây bệnh đốm lá bằng hỗn hợp hệ nano của tinh dầu ớt, tinh dầu tỏi và tinh dầu sả chanh

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Tác giả: Minh Hiệp Nguyễn, Ngọc Thùy Trang Nguyễn, Thị Huỳnh Nga Nguyễn, Thị Ngọc Mai Trần, Ngọc Bích Đào Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 641.6385 Cooking specific materials

Thông tin xuất bản: Khoa học và Công nghệ Nông nghiệp Việt Nam, 2020

Mô tả vật lý: 141 - 145

Bộ sưu tập: Metadata

ID: 493941

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Nghiên cứu nhằm mục đích đánh giá hiệu quả ức chế nấm Alternaria sp. gây bệnh đốm lá cùa các tinh dầu thực vật ở dạng nano trong điều kiện in vitro. Đầu tiên, tinh dầu thực vật (ớt, tỏi và sả chanh) được đóng gói vào hệ mang nano băng phương pháp đồng hóa tốc độ cao kết hợp với sóng siêu âm để giảm kích thước, hỗn hợp được tạo thành khi trộn ba dịch phân tán nano của tinh dầu tỏi, ớt và sả chanh. Sau đó, hiệu quả kháng nấm in vitro cùa các hệ nano chứa tinh dấu thực vật (dạng đơn lẻ và hỗn hợp) được thực hiện bằng phương pháp đĩa thạch ở các nồng độ pha loãng khác nhau 250, 500, 750 và 1000 lần. Kết quả cho thấy, các hệ nano tinh dầu đã được tổng hợp thành công với kích thước hạt trung bình nhỏ hơn 151 nm và thế zeta lớn hơn -30 mV. Kết quả cùng cho thấy sự kết hợp giữa ba hệ nano chứa tinh dầu ớt, tinh dầu tỏi và tinh dầu sà chanh vào trong một chế phẩm theo tỉ lệ 1 : 1 : 1 (v/v/v) (gọi tắt là naOTS) cho hiệu quả ức chế nấm Alternaria sp. trong điều kiện in vitro là cao nhất (đạt 97,78% ở nồng độ pha loãng 750 lần). Do đó, naOTS có tiềm năng ứng dụng rộng rãi thay thế cho các thuốc trừ nấm hóa học trong phòng trừ nấm Alternaria sp.This study aims to investigate the in vitro inhibitory effect ofnano-formulations ofessential oils against Alternaria sp. causing leafspot disease Firstly, essential oil (chili oil, garlic oil and lemongrass oil) encapsulated nanocarriers was prepared by using a combing method ofhomogenization and sonication, and their mixture was generated by mixing these 3 nanoformulations. After that, the in vitro antifungal effect of these prepared nano-formulations (individual forms and mixture forms) was carried out by agar disk method at 250, 500, 750, and 1000 times dilution. The results showed that the nano-formulations of essential oils were successfully prepared with an average particle size smaller than 151 nm and the zeta potential lager than -30 mV. The results also indicated that the mixture with ratio 1:1:1 (v/v/v) ofthe nano-formulations ofchili oil, garlic oil and lemongrass oil (NaOTS) had the highest in vitro inhibitory effectiveness (approximately 97.78% at 750 times dilution) on Alternaria sp. Therefore, NaOTS shows great potential ofwide application for replacing chemical fungicides in Alternaria sp. control.

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