Nghiên cứu xây dựng quy trình tích hợp đa gen kháng đa yếu tố vào giống lúa chất lượng bằng chỉ thị phân tử (mabc)

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Tác giả: Đức Hà Chu, Văn Khởi Đào, Hùng Lĩnh Lê, Bá Ngọc Nguyễn, Thị Minh Nguyệt Nguyễn, Thị Nhài Nguyễn, Thị Thanh Thủy Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 571.962 Physiology and related subjects

Thông tin xuất bản: Nông nghiệp & phát triển nông thôn, 2021

Mô tả vật lý: 45363

Bộ sưu tập: Metadata

ID: 494434

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A high quality rice cultivar Bac Thom No. 7 (BT7) is one of the most popular rice varieties in the Northern provinces. However, cultivation of BT7 is seriously affected by the development of pests and diseases, especially bacterial leaf blight, blast and brown plant hopper. This study is carried out with the purpose of building the procedure of introgressing multiple resistance genes for multi-traits into high quality rice cultivar by using marker-assisted backrossing (MABC). The research results have successfully applied the MABC method to introgress 5-6 resistance genes (xa5, Xa7, Bph20, Bph21, Pil and Pi9 (Ợ) into the high quality rice cultivar, Bac Thom No7 (BT7). A potential rice line, AGI-6 (from the original line BT7.10.20) harboring 6 resistance genes xa5, Xa7, Bph20, Bph21, Pil and P19 (t), demonstrated good tolerance to target traits: resistant to bacterial leaf blight (score 2.9), middle resistant to brown plant hopper (score 3.7), and resistant to blast (score 3.0). AGI-6 line showed the main agronomic characteristics of original variety BT7, grain quality was almost similar to BT7 and yield 14.7% higher than BT7 in the summer crop. This line will be further selected for purification and sent for VCU, DUS testing and production trials in ecological regions to evaluate the adaptability and prospect of new variety in production. "Procedure of introgressing multiple resistance genes for multi-traits into high quality rice cultivar by using marker-assisted backrossing (MABC)" can be applied to improve plant varieties with desirable traits for sustainable agricultural production in Vietnam.Giống lúa chất lượng Bắc Thơm số 7 (BT7) là một trong những giống lúa chủ lực trong sản xuất lúa gạo ở các tỉnh phía Bắc. Tuy nhiên, canh tác giống BT7 đang chịu ảnh hưởng nghiêm trọng do sự phát triển của sâu bệnh hại, đặc biệt là bệnh bạc lá, bệnh đạo ôn và rầy nâu. Nghiên cứu này được thực hiện với mục đích xây dựng Quy trình tích hợp đa gen kháng đa yếu tố vào giống lúa chất lượng bằng chỉ thị phân tử (MABC). Kết quả nghiên cứu đã áp dụng thành công phương pháp chọn giống dựa vào chỉ thị phân tử và lai trở lại (MABC) để tích hợp được 5-6 gen kháng (bệnh bạc lá, rầy nâu và đạo ôn) vào giống lúa chất lượng BT7. Dòng lúa triển vọng AGI-6 (từ dòng gốc BT7.10.20) tích hợp 6 gen kháng xa5, Xa7, Bph20, Bph21, Pil và Pi9(t) đã biểu hiện khả năng chống chịu tốt với các tính trạng mục tiêu: kháng bạc lá điểm 2,9, khảng vừa rầy nâu điểm 3,7 và kháng đạo ôn điểm 3,0. Dòng AGI-6 mang các đặc điểm nông sinh học chính của giống gốc BT7, có chất lượng cơm gạo gần tương tự giống gốc BT7 và có năng suất vượt đối chứng 14,7% trong vụ mùa sê được tiếp tục chọn lọc làm thuần và gửi khảo nghiệm vcu, khảo nghiệm DUS và khảo nghiệm sản xuất tại các vùng sinh thái để đánh giá khả năng thích ứng và triển vọng của giống lúa tích hợp đa gen kháng. "Quy trình tích hợp đa gen kháng đa yếu tố vào giống lúa chất lượng bàng chỉ thị phân tử ABC của khả năng áp dụng để cải tiến giống cây trồng mang những tính trạng mong muốn phục vụ sản xuất nông nghiệp bền vững tại Việt Nam.

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