Xác định đặc điểm vùng gen rbcL và trnH-psbA của phân loài Vân sam Phan Xi Păng (Abies delavayi subsp. fansipanensis (Q.P. Xiang) Rurhforth) ở Việt Nam

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Tác giả: Thị Thu Hằng Lại, Hùng Mạnh Nguyễn, Thị Hồng Mai Nguyễn, Thị Phương Trang Nguyễn, Văn Sinh Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 610.915 Medicine and health

Thông tin xuất bản: Khoa học & công nghệ Việt Nam, 2021

Mô tả vật lý: 28-32

Bộ sưu tập: Metadata

ID: 494511

Thêm vào giỏ

Abies delavayi subsp. fansipanensis (Xiang Q.P.) Rurhforth) or Abies delavayi var. nukiangensis auct. non is a native plant, distributes at altitudes about 2,400 m above sea level. This plant belongs to the Pinaceae family and is listed in endangered, precious and rare species banned from commercial exploitation. The Abies delavayi subsp. fansipanensis population in Vietnam only concentrates in a mountainous area at the peak of Fansipan with a population diameter of about 3 km at an altitude of 2,600 to 2,950 m, and is about 500 km far from the Abies delavayi population in Cang Shan (China). In this study, the author sequenced the region of the rbcL and trnH-psbA of Abies delavayi subsp. fansipanensis in Vietnam, compared with the Abies delavayi of China to better understand the genetic characteristics of Abies delavayi subsp. fansipanensis in Vietnam. The results showed that the Abies delavayi subsp. fansipanensis in Vietnam has a different nucleotide position compared to the Chinese Abies nukiangensis at nucleotide no. 455 on the rbcL gene region and two other nucleotide positions in the trnH-psbA genome at positions 332 and 503. The sequences of the rbcL and trnH-psbA gene regions of Abies delavayi subsp. fansipanensis from Vietnam were registered into the GenBank with the accession number MK783132 and MK783131, respectively.Vân sam Phan Xi Păng (Abies delavayi subsp. fansipanensis (Q.P. Xiang) Rurhforth) là loài thực vật bản địa thường phân bố ở độ cao từ 2.400 m so với mực nước biển. Đây là loài nằm trong danh lục các loài thực vật nguy cấp cần được bảo vệ và bị cấm khai thác, sử dụng vì mục đích thương mại. Quần thể Vân sam của Việt Nam tập trung duy nhất ở một vùng núi thuộc đỉnh Phan Xi Păng với đường kính quần thể khoảng 3 km, ở độ cao 2.600-2.950 m, cách xa quần thể Vân sam ở Cang Shan, Trung Quốc khoảng 500 km. Trong nghiên cứu này, nhằm tìm hiểu rõ hơn về sự sai khác di truyền giữa loài Vân sam phân bố ở Phan Xi Păng Việt Nam với loài Vân sam phân bố tại Trung Quốc, các tác giả đã tiến hành đọc trình tự 2 vùng gen lục lạp gồm rbcL và trnH-psbA và so sánh với loài Vân sam của Trung Quốc. Kết quả cho thấy, phân loài Vân sam Phan Xi Păng của Việt Nam có một vị trí Nucleotide sai khác so với loài Abies nukiangensis của Trung Quốc ở vị trí số 455 trên vùng gen rbcL và 2 vị trí Nucleotide sai khác trên vùng gen trnH-psbA ở vị trí số 332 và 503. Trình tự hai vùng gen rbcL và trnH-psbA của loài Vân sam Phan Xi Păng của Việt Nam đã được đăng ký lên Ngân hàng gen thế giới (GenBank) với mã số truy cập là MK783132 và MK783131.

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