In this study, ultra-high pressure (UHP) and heat-assisted technology (HT) were used to process walnut peptides (WP) and investigate their combined effects (UHP-HT) on the characterization, moisture absorption, and antioxidant activity of WP. The results indicated that UHP (300 Mpa, 10 min) combined with HT treatment (55 °C, 30 min) significantly increased the surface hydrophobicity and disulfide bonds of WP. UHP-HT-treated WP exhibited lower moisture absorption and more stable water molecule migration. Additionally, the moisture absorption capacity of the WP (48.78 %) was significantly decreased in WP-UHP, WP-HT and WP-UHP (45.37 %, 43.15 %, and 40.19 %, respectively) because of increasing the surface hydrophobicity. UHP-HT combined improved structural characteristics, including particle size, zeta potential, and functional group stability, and significantly enhanced the antioxidant activity of WP under high humidity conditions. Overall, these findings suggest that UHP-HT can effectively reduce the moisture absorption of WP, thus enhancing its storage stability and extending its shelf life.