Một số đặc điểm di truyền các vùng kháng nguyên và một số vị trí epitope liên kết với kháng thể trung hòa đơn dòng trên glycoprotein của các vi rút dại phân lập tại Việt Nam, 2015-2019

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Tác giả: Đức Anh Đặng, Châu Giang Ngô, Qúy Lâm Ngô, Hoàng Trung Nguyễn, Huy Điện Nguyễn, Thị Kiều Anh Nguyễn, Tuyết Thu Nguyễn, Văn Chuyên Nguyễn, Văn Khải Nguyễn, Vĩnh Đông Nguyễn, Inoue Satoshi

Ngôn ngữ: vie

Ký hiệu phân loại: 571.9648 Diseases Pathology

Thông tin xuất bản: Y học dự phòng, 2020

Mô tả vật lý: 74-86

Bộ sưu tập: Metadata

ID: 496331

Thêm vào giỏ

Nghiên cứu mô tả đặc điểm di truyền và kháng nguyên vùng ectodomain trên protein G của vi rút dại phân lập tại Việt Nam. Nghiên cứu đã phân tích trình tự acid amin các ectodomain (mang các vị trí quyết định kháng nguyên chủ yếu) tại glycoprotein (protein G) của 14 chủng vi rút dại phân lập gần đây tại một số tỉnh miền Bắc, miền Trung và miền Nam Việt Nam. Trình tự acid amin vùng ectodomain của protein G giữa các vi rút dại hoang dại ở Việt Nam và các chủng vắc xin phổ biến có mức độ tương đồng từ 89,7% đến 91,4%, cao hơn đáng kể so với mức tương đồng acid amin 74% (ngưỡng bảo vệ chéo của vắc xin). Sự bảo tồn cao của hầu hết các vị trí kháng nguyên giữa các chủng vi rút dại hoang dại và các vắc xin phổ biến cũng quan sát thấy trong nghiên cứu. Các vị trí tương tác kháng nguyên - kháng thể (epitope) liên kết với một số kháng thể trung hòa đơn dòng (Mab) quan trọng được đề xuất thay thế immunoglobulin (RIG) trong điều trị sau phơi nhiễm (PEP) đối với bệnh dại đã được khảo sát trong nghiên cứu, kết quả không phát hiện bất cứ đột biến acid amin dẫn đến làm giảm hoặc loại bỏ khả năng trung hòa của kháng thể ở tất cả các trình tự được phân tích. Nghiên cứu cho thấy các vắc xin thương mại hiện nay vẫn có khả năng bảo vệ tốt đối với chủng dại hoang dại cũng như khả năng sử dụng một số loại Mab cho điều trị dự phòng bệnh dại ở Việt Nam.This study aims to describe genetic characterristics of the antigenic sites and some binding epitopes for monoclonal antibodies on the glycoprotein of rabies virus isolated in Vietnam. In the present study, sequences of the ectodomains (which carries the main antigenic sites) of glycoprotein from 14 rabies virus representing for two rabies virus groups circulating in Viet Nam recently were analyzed. Comparing the ectodomain amino acid sequence of the protein G rabies strains in Vietnam and popular vaccine strains, the similarity ranged from 89.7% to 91.4%, signifcantly higher than the cut oﬀ of amino acid identity 74% (vaccine cross-neutralization). Furthermore, the conservation of most of the antigenic sites between the rabies virus strains was also found in our study and the popular vaccines strains. The conservation of binding epitopes for several well characterized rabies virusneutralizing Mabs proposed for a replacement of conventional rabies immunoglobulin (RIG) in human PEP was also determined. The results showed that the binding epitopes for Mabs using in the study were highly conserved. The critical amino acid substitutions diminishing or abolishing the binding of these Mabs were not detected. All together, these results collectively demonstrated that the available commercial vaccines are still protective against the rabies strains circulating in Vietnam and suggest that some certain MAbs can be considered as good candidates for a cocktail Mabs for Passive immunization against rabies in Vietnam.

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