OBJECTIVE: This study aimed to investigate the resin compounds from CAD-CAM 3D-printed denture resins, focusing on the identification and classification of free monomers and other components. The primary objective was to determine the chemical profile of these 3D-prinding resin materials. METHODS: Four 3D-printed denture resins, two base materials (1: DentaBASE, Asiga Ltd., Alexandria, NSW, Australia
2: DENTURETEC, SAREMCO Dental AG), and two tooth-colored materials (1: DentaTOOTH, Asiga Ltd.
2: CROWNTEC (SAREMCO Dental AG), were analyzed using high-performance liquid chromatography and mass spectrometry (HPLC-MS). Data analysis was processed including peak alignment and normalization to an ethanol blank, resulting in detailed identification of compounds in the resin. RESULTS: Out of 5,208 detected compounds in the resin, 63 were retained after applying filtering criteria for further analysis, categorized into methyl methacrylate (MMA) and derivatives (31), photo initiators (8), UV stabilizers (1), and other additives (23). Sample 2B exhibited the highest number of resin compounds (62), including the most MMA derivatives and additives. MMA accounted for over 69% in abundance across samples, with unique additives such as ethyl-4-dimethylaminobenzoate and bis(acryloyloxymethyl) tricyclo[5.2.1.02,6] decane (TCD-DI-HEA) identified. Variations in the resin composition distribution and abundance highlighted differences in resin formulations. CONCLUSIONS: Within the limits of this in vitro, exploratory study, unpolymerized 3D-printed denture resins were analyzed, and various resin compounds in 3D-printed denture resins, including MMA, photo initiators, UV stabilizers, and additives, were identified along with significant variability between samples. While 3D-printing enhances efficiency and customization, further investigation is needed to better understand and assess the long-term intraoral effects and their effectiveness in complete dentures. CLINICAL SIGNIFICANCE: This study provides insights into the chemical composition of 3D-printed denture resins, highlighting variability in resin compound profiles. Understanding these materials is essential for ensuring biocompatibility and performance. The findings support the need for further research and standardized testing to guide clinicians in the safe and effective use of 3D-printed dentures.