In vitro fertilization (IVF) technology for embryo production has been applied in basic research, animal husbandry and medicine. However, the developmental efficiency and quality of embryos produced by IVF are inferior to those produced in vivo. In this study, we investigated the effects of supplementing bovine oviductal epithelial cells (BOEC) lysate during the in vitro culture period on the developmental competence and quality of bovine embryos. IVF embryos were cultured for 4 days post-IVF in medium supplemented with 10 % BOEC lysate at various concentrations (1.0 × 10