Khảo sát phát hiện escherichia coli sinh new delhi metallo-β-lactamase (blandm-1) trên chó khỏe mạnh ở các trại thú cưng Đắk Lắk

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Tác giả: Mạnh Hùng Đặng, Ian Campbell James, Chí Kiên Lê, Thị Ngọc Huyền Nguyễn, Văn Minh Hoàng Nguyễn, Thị Phượng Trang Phan, Thanh Xuân Trần, Thị Bích Chiêu Trần, Quốc Cường Võ

Ngôn ngữ: vie

Ký hiệu phân loại: 636.76 Toy dogs

Thông tin xuất bản: Tạp chí Khoa học - Đại học Sư phạm TP Hồ Chí Minh, 2021

Mô tả vật lý: 976-986

Bộ sưu tập: Metadata

ID: 498011

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Carbapenem antibiotic, which was presented during 1980s, have acted as the last line of defense against multidrug-resistant Gram-negative organisms. The occurence of E. coli carrying the new carbapenemase: New Delhi metallo-β-lactamase (blaNDM-1) in human has been known for many years. However, limited studies have been conducted on animal in Viet Nam. This study aimed to investigate the antimicrobial susceptibility testing of E. Coli and evaluate the phenotype and genotype of carbapenem-resistance E. coli. About 269 E. coli isolates were isolated from sixty-four rectal swabs in dogs to check the antimicrobial susceptibility testing (AST) for different 11 types of antimicrobials. In this study, we combined modified carbapenem inactivation method (mCIM) and EDTA-modified carbapenem inactivation method EDTA-CIM (eCIM) for phenotypic detection of carbapenemase producing E. coli and Polymerase Chain reaction method (PCR) for detecting and distinguishing three main types of the metallo-β-lactamases genes (NDM, VIM, IMP). The result of antimcrobial susceptibility testing by Kirby-Bauer method showed that 4/269 isolates had resistance to Carbapenem, 1/4 isolate was detected with metallo-β-lactamase by mCIM and eCIM and confirmed with NDM-1 gene by PCR. These findings provide up-to-date information on the first detection of E. coli carrying resistance genes (blaNDM-1) in healthy dogs in Vietnam.Từ những năm 80 của thế kỉ XX, carbapnemase được coi là vũ khí cuối cùng để chống lại các vi khuẩn Gram âm đa kháng thuốc. Việc phát hiện các chủng vi khuẩn E. coli mang gen kháng kháng sinh mới: New Delhi metallo-β-lactamase (blaNDM-1) trên người đã được biết đến trong những năm qua
tuy nhiên, rất ít các nghiên cứu được tiến hành trên động vật tại Việt Nam. Mục đích của nghiên cứu là khảo sát tính đề kháng và đánh giá kiểu hình, kiểu gen của các chủng E. coli kháng carbapenem trên chó khỏe mạnh ở Đắk Lắk. Khoảng 269 chủng E. coli được phân lập từ 64 mẫu phết hậu môn trên chó, được tiến hành khảo sát đề kháng bằng phương pháp định tính Kirby-Bauer với 11 loại kháng sinh khác nhau. Sự kết hợp của phương pháp bất hoạt Carbapenem cải tiến (mCIM) và EDTA - Carbapenem cải tiến EDTA-CIM (eCIM) để phát hiện kiểu hình E. coli sản sinh carbapenemase và PCR được thực hiện để xác định các gen kháng metallo-β-lactamases (NDM, VIM, IMP). Kết quả nghiên cứu phát hiện 4/269 chủng E. coli kháng với Carbapenem, 1/4 chủng phát hiện metallo-β-lactamase dựa trên kiểu hình mCIM và eCIM, dương tính với NDM-1 trên kiểu gen bằng PCR. Nghiên cứu mang ý nghĩa cập nhật thông tin cao bởi đây là phát hiện E. coli mang gen kháng NDM-1 trên chó khỏe mạnh đầu tiên tại Việt Nam.

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