End-of-life (EoL) reverse osmosis (RO) membranes were regenerated by an extended alkaline cleaning treatment (ACT) followed by polyelectrolyte (PE) deposition (coating). The effect of time delay between the ACT and PE coating on the membranes' stability and filtration properties was investigated. The permeance of the membranes increased more than twofold compared to the value exhibited by the EoL membrane before the ACT. Additionally, the surface charge decreased from -45 mV to -99 mV at pH 7.7, due to the ACT. However, the ACT-induced effects were predominantly time-dependent and were partially reversed over time. When the membrane was coated with one layer of polydiallyldimethylammonium chloride (PDADMAC) immediately after the ACT, the resulting membrane had approximately 800 g/mol molecular weight cut-off (MWCO) value and 30 L/(m