Xác định tỷ lệ nhiễm và yếu tố độc lực của vi khuẩn Salmonella phân lập ở lợn nuôi tại huyện Hiệp Hòa, tỉnh Bắc Giang, Việt Nam=The prevalence and virulence of Salmonella isolates from pigs in Hiep Hoa district, Bac Giang province, Viet Nam

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Tác giả: Bình Đặng Xuân, Linh Nguyễn Thị Thùy

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.26

Bộ sưu tập: Metadata

ID: 498309

Thêm vào giỏ

Nghiên cứu đã được thực hiện nhằm xác định tỷ lệ nhiễm và độc lực của vi khuẩn Salmonella thải trừ từ 256 lợn nái nuôi ở 20 trang trại thuộc huyện Hiệp Hòa, tỉnh Bắc Giang. Kết quả phân tích các mẫu bệnh phẩm cho thấy có 2/166 mẫu dương tính với Salmonella weltevreden
3/166 mẫu dương tính với Salmonella dublin
5/166 mẫu dương tính với Salmonella anatum và Salmonella senftenberg
6/166 mẫu dương tính với Salmonella heidelberg
9/166 mẫu dương tính với Salmonella enteritidis
30/166 mẫu dương tính với Salmonella typhimurium, 41/166 mẫu dương tính với Salmonella choleraesuis, 10/166 mẫu dương tính với Salmonella chưa rõ serotype. Các chủng vi khuẩn Salmonella phân lập được mang gen mã hóa yếu tố độc lực, thể hiện tính gây bệnh, bao gồm: tỷ lệ các chủng Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis mang gen mã hóa độc tố đường ruột chịu nhiệt Stn lần lượt là 73,3%, 88,8%, 92,6%
Tỷ lệ các chủng Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis mang gen fimA lần lượt là 66,6%, 88,8%, 92,6%
Tỷ lệ các chủng Salmonella typhimurium, Salmonella choleraesuis, Salmonella enteritidis mang gen InvA lần lượt là 26,6%, 39,0%, 66,6%. Tỷ lệ chủng Salmonella choleraesuis kháng nalidixic acid là 2,4%
kháng ciprofloxacin, rifampicin, spectinomycin là 7,3%
kháng ceftazidime, oxytetracycline là 9,7%
kháng nitrofurantoin là 12,1%
kháng trimethoprim-sulfamethoxazole là 19,5%
kháng kanamycin là 21,9%. Tỷ lệ chủng Salmonella enteritidis kháng ciprofloxacin, rifampicin, ceftazidime, spectinomycin, nitrofurantoin là 11,1%
kháng trimethoprim-sulfamethoxazole, kanamycin là 22,2%. Tỷ lệ chủng Salmonella typhimurium kháng nitrofurantoin, nalidixic acid và ceftazidime là 3,3%
kháng ciprofloxacin, spectinomycin và rifampicin là 6,6%
kháng trimethoprim-sulfamethoxazole là 16,6%
kháng kanamycin là 20,0%. Các chủng Salmonella phân lập được mẫn cảm mạnh với nalidixic acid, oxytetracycline, ceftazidime, rifampicin, ciprofloxacin và nitrofurantoin
đồng thời biểu hiện kháng lại các kháng sinh này ở mức độ khác nhau, chiếm tỷ lệ cao nhất là trimethoprim-sulfamethoxazole, spectinomycin và kanamycin.The study was conducted to determine the infection rate and virulent factors of Salmonella bacteria isolating from 256 sows in 20 breeding farms in Hiep Hoa district, Bac Giang province, Viet Nam. The studied result showed that the serotype of 2/166 positive samples with Salmonella weltevreden
3/166 positive samples with Salmonella dublin
5/166 positive samples with Salmonella anatum and Salmonella senftenberg
6/166 positive samples with Salmonella heidelberg
9/166 positive samples with Salmonella enteritidis
30/166 positive samples with Salmonella typhimurium, 41/166 positive samples with Salmonella choleraesuis, and 10/166 positive samples with Salmonella were unknown. The isolated Salmonella strains carried the virulent genes, including the Salmonella typhimurium, Salmonella enteritidis and Salmonella choleraesuis strains bearing Stn gene (causing enteritidis) accounted for 73.3%, 88.8%, and 92.6%, respectively. The Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis strains bearing fimA gene accounted for 66.6%, 88.8%, and 92.6%, respectively. The Salmonella typhimurium, Salmonella choleraesuis and Salmonella enteritidis strains bearing InvA gene accounted for 26.6%, 39.0%, and 66.6%, respectively. The rate of Salmonella choleraesuis strain resisting nalidixic acid
ciprofloxacin, rifampicin, spectinomycin
ceftazidime, oxytetracycline
nitrofurantoin
trimethoprim-sulfamethoxazole
kanamycin was 2.4%, 7.3%, 9.7%, 12.1%, respectively. The rate of Salmonella enteritidis strain resisting ciprofloxacin, rifampicin, ceftazidime, spectinomycin, nitrofurantoin
trimethoprimsulfamethoxazole, kanamycin was 11.1%, 22.2%, respectively. The rate of Salmonella typhimurium strain resisting nitrofurantoin, nalidixic acid and ceftazidime
ciprofloxacin, spectinomycin and rifampicin
trimethoprim-sulfamethoxazole
kanamycin was 3.3%, 6.6%, 16.6%, 20.0%, respectively. The isolated Salmonella strains were strongly susceptible with nalidixic acid, oxytetracycline, ceftazidime, rifampicin, ciprofloxacin and nitrofurantoin. They also resisted to the above mentioned antibiotics at various levels. Of which, resistance to trimethoprim-sulfamethoxazole, spectinomycin, and kanamycin accounted for the highest rate.

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