Development of an efficient differentiation culture system of murine HSC into megakaryocytes.

• Tác giả: Yue Chai, Fang Dong, Hideo Ema, Toshio Suda, Chong Yang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 338.9 Economic development and growth
• Thông tin xuất bản: United States : Biochemical and biophysical research communications , 2025
• Bộ sưu tập: NCBI
• ID: 498322

Tóm tắt

Despite significant progress in the cultivation of hematopoietic stem cells (HSCs), the establishment of lineage-specific cell culture systems remains inadequately developed. This study compares the effects of recombinant human serum albumin (r-HSA) and polyvinyl alcohol (PVA) in serum-free culture systems on the differentiation of HSCs into multiple lineages. Both single-cell and multi-cellular culture systems are used, and differentiation is evaluated by flow cytometry and slides-based techniques under various cytokine conditions. Our results show that r-HSA strongly promotes differentiation into megakaryocytes (MKs) compared to PVA. The findings indicate that r-HSA outperforms PVA in supporting MK differentiation through both early expansion and later differentiation. This study provides insights into optimizing megakaryocyte generation and offers a more effective culture system for clinical applications.