Nghiên cứu phân lập và giải trình tự gen virus đậu trên dê ở Việt Nam=Isolation and gene sequence analysis of goat pox virus in Viet Nam

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Tác giả: Nguyễn Thị Hoa, Huyên Nguyễn Thị, Trương Quang Lâm, Lan Hương Lại Thị, Lan Nguyễn Thị, Nguyễn Thị Yến

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.5

Bộ sưu tập: Metadata

ID: 498505

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A total of ninety goats suspecting infection with goat pox disease were collected from BaVi - Ha Noi, Ninh Binh, Hoa Binh and Nghe An provinces for this study. By using polymerasechain reaction (PCR) technique for identifying the positive samples, 72 goats were found to bepositive with goat pox virus, accounting for 80% of the total studied animals. The lung and pustulesamples were collected from the positive goats for isolating goat pox virus in primary lamb testiscells. There were 7 different goat pox virus strains were successfully isolated. Based on biologicalproperties, the 5 most virulent virus strains including GTPV-BV1, GTPV-HB1, GTPV-NA1, GTPVNB1, GTPV-NB2 were selected and gene sequences of these virus strains were analysed. Theidentity/similarity level of amino acid and nucleotide sequences of 5 virus strains reached 81.1 to95.99% and 90.1 to 98.38%, respectively. The result of phylogenetic analysis revealed that thesestrains belonged to the same genotype. Remarkably, the GTPV- HB1 isolate was classified intosub-lineage which closely related with goat pox virus strains from China, America and vaccinestrain AY077836/G20-LKVNghiên cứu đã được tiến hành trên 90 con dê nghi mắc bệnh đậu, thu thập tại Ba Vì, Hà Nội, NinhBình, Hòa Bình và Nghệ An. Bằng phương pháp PCR với các mẫu dê thu thập để phát hiện virus đậudê, đã phát hiện được 72 con dê dương tính với virus đậu, chiếm tỷ lệ 80%. Từ các mẫu dê dương tính,đã lấy những mẫu phổi hoặc mụn đậu rồi tiến hành phân lập virus đậu dê trên tế bào tinh hoàn cừu sơcấp. Kết quả đã phân lập thành công 7 chủng virus đậu dê từ số lượng mẫu dê và địa điểm thu mẫu nêutrên. Nghiên cứu đặc tính sinh học của 7 chủng virus này, đã chọn ra được 5 chủng có hiệu giá cao, đólà GTPV-BV1, GTPV-HB1, GTPV-NA1, GTPV-NB1, GTPV-NB2, và đã tiến hành giải trình tự gencủa chúng. Kết quả phân tích mức độ tương đồng về trình tự nucleotide và amino acid của 5 chủngnghiên cứu cho thấy mức tương đồng về trình tự nucleotide đạt 90,1% - 98,38% và mức tương đồngvề trình tự amino acid đạt 81,1% - 95,99%. Kết quả phân tích cây sinh học phân tử cho thấy 5 chủngnghiên cứu thuộc cùng 1 nhánh phát sinh. Đáng chú ý là chủng phân lập GTPV-HB1 ở cùng nhánhvới các chủng phân lập tại Trung Quốc, Mỹ và chủng vacxin AY077836/G20-LKV.

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